| Literature DB >> 4827918 |
T T Ishikawa, J MacGee, J A Morrison, C J Glueck.
Abstract
A gas-liquid chromatographic micromethod for quantitation of cholesterol in 20 micro l of plasma was developed using 5alpha-cholestane as an internal standard, saponification with tetramethylammonium hydroxide-isopropanol, and extraction with tetrachloroethylene-methyl butyrate. Cholesterol levels in plasma samples were calculated by comparing cholesterol-cholestane peak height ratios with those of preassayed reference plasma. Over a plasma cholesterol range of 44 to 468 mg/100 ml, the gas-liquid chromatographic micromethod and the automated ferric chloride colorimetric method gave nearly identical results (r = 0.99) in duplicate aliquots of 131 plasma samples.Entities:
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Year: 1974 PMID： 4827918
Source DB: PubMed Journal: J Lipid Res ISSN： 0022-2275 Impact factor: 5.922