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Literature DB >> 4822735

Contamination of free-ribosome fractions by detached previously membrane-bound ribosomes.

Abstract

A rough-membrane fraction isolated from rat liver by a procedure designed to prevent membrane denaturation was subjected to the gradient treatment normally used to isolate free ribosomes. Under these conditions, at most 20% of the ribosomes were detached from membrane with less than 5% sedimenting into the free-polyribosome pellet.

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RNA

Year: 1974 PMID： 4822735 PMCID： PMC1166208 DOI： 10.1042/bj1380305

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

12 in total

1. The influence of the physical state of microsomal membranes from rat liver on translational processes of polyribosomes.

Authors: W S Bont; J Geels; A Huizinga; K Mekkelholt; P Emmelot
Journal: Biochim Biophys Acta Date: 1972-04-12

2. Studies on the nature of attachment of ribosomes to membranes in liver. I. Influence of ethionine, sparsomycin, CCl 4 , and puromycin on membrane-bound polyribosomal disaggregation and on detachment of membrane-bound ribosomes from membranes.

Authors: D S Sarma; I M Reid; E Verney; H Sidransky
Journal: Lab Invest Date: 1972-07 Impact factor: 5.662

Contamination of free-ribosome fractions by detached previously membrane-bound ribosomes.

1. The influence of the physical state of microsomal membranes from rat liver on translational processes of polyribosomes.

2. Studies on the nature of attachment of ribosomes to membranes in liver. I. Influence of ethionine, sparsomycin, CCl 4 , and puromycin on membrane-bound polyribosomal disaggregation and on detachment of membrane-bound ribosomes from membranes.

3. Ribosome monomers in rat liver following administration of dimethylnitrosamine.

Review 4. Recent developments in the measurement of nucleic acids in biological materials. A supplementary review.

5. Biosynthesis of serum proteins and ferritin by free and attached ribosomes of rat liver.

6. Free and membrane-bound ribosomes in rat liver.

7. Electron microscopic observation on polyribosomes and endoplasmic reticulum fragments isolated from rat liver.

8. Studies on free and membrane-bound ribosomes in rat liver. I. Distribution as related to total cellular RNA.

9. Isolation and properties of polyribosomes and fragments of the endoplasmic reticulum from rat liver.

10. Protein synthesis by membrane-bound and free ribosomes of secretory and non-secretory tissues.

1. Polyribosomes from Peas: V. An Attempt to Characterize the Total Free and Membrane-bound Polysomal Population.

2. The binding of monoribosomes, oligoribosomes and polyribosomes to reticular membranes from rat liver.