Literature DB >> 4816300

The effect of 2-mercaptoethanol on murine mixed lymphocyte cultures.

M J Bevan, R Epstein, M Cohn.   

Abstract

Mouse spleen cells which have been depleted of adherent cells do not respond to allogeneic lymphocytes in vitro. Their cytotoxic response can be restored by inclusion of mercaptoethanol in the medium. Mercaptoethanol is shown to have a stimulatory effect also on the response of normal (unseparated) spleen cells to alloantigens. The enhancement of the DNA-synthetic and cytotoxic response is similar, varying from 3.5-15-fold. Cytotoxic cells also appear in unmixed lymphocyte cultures in the presence of mercaptoethanol and fetal calf serum. The specificity of these background cytotoxic cells is not known.

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Year:  1974        PMID： 4816300      PMCID： PMC2139578          DOI： 10.1084/jem.139.4.1025

Source DB:  PubMed          Journal:  J Exp Med        ISSN： 0022-1007            Impact factor:   14.307


  16 in total

1.  Mixed lymphocyte culture and HL-A.

Authors:  V P Eijsvoogel; L de Groot-Kooy; L Huismans; J J van Rood; A van Leeuwen; E D DuToit
Journal:  Transplant Proc       Date:  1972-06       Impact factor: 1.066

2.  Effect of column purification on response of lymphocytes in mixed leucocyte cultures (MLC) and to blastogenic media deprived from MLCS.

Authors:  A L Jones
Journal:  Clin Exp Immunol       Date:  1971-06       Impact factor: 4.330

3.  Role of monocytes in the mixed leukocyte culture reaction.

Authors:  J Gordon
Journal:  Proc Soc Exp Biol Med       Date:  1968-01

4.  The mixed leukocyte culture: a three component system.

Authors:  H N Rode; J Gordon
Journal:  J Immunol       Date:  1970-06       Impact factor: 5.422

5.  Cellular requirements for the mitotic response in allogeneic mixed leukocyte cultures.

Authors:  J J Twomey; O Sharkey; J A Brown; A H Laughter; P H Jordan
Journal:  J Immunol       Date:  1970-04       Impact factor: 5.422

6.  Enhancement by reducing agents of the transformation of human and rabbit peripheral lymphocytes.

Authors:  M W Fanger; D A Hart; J V Wells; A Nisonoff
Journal:  J Immunol       Date:  1970-10       Impact factor: 5.422

7.  Quantitative in vitro assay of cytotoxic cellular immunity.

Authors:  T G Canty; J R Wunderlich
Journal:  J Natl Cancer Inst       Date:  1970-10       Impact factor: 13.506

8.  The effects of mercaptoethanol and of peritoneal macrophages on the antibody-forming capacity of nonadherent mouse spleen cells in vitro.

Authors:  C Chen; J G Hirsch
Journal:  J Exp Med       Date:  1972-09-01       Impact factor: 14.307

9.  Cell-mediated immune response in vitro. 3. The requirement for macrophages in cytotoxic reactions against cell-bound and subcellular alloantigens.

Authors:  H Wagner; M Feldmann; W Boyle; J W Schrader
Journal:  J Exp Med       Date:  1972-08-01       Impact factor: 14.307

10.  Immunization of dissociated spleen cell cultures from normal mice.

Authors:  R I Mishell; R W Dutton
Journal:  J Exp Med       Date:  1967-09-01       Impact factor: 14.307
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  20 in total

1.  Immune responses of diabetic animals. Direct immunosuppressant effects of streptozotocin in mice.

Authors:  W K Nichols; J B Spellman; L L Vann; R A Daynes
Journal:  Diabetologia       Date:  1979-01       Impact factor: 10.122

2.  Modification of lymphocyte responsiveness in vitro by lambda carrageenan compared with colloidal silica and depletion of surface adherent cells.

Authors:  G Pawelec; G Brons
Journal:  Clin Exp Immunol       Date:  1978-03       Impact factor: 4.330

3.  Differences in the surface proteins of mouse B and T cells.

Authors:  I S Trowbridge; P Ralph; M J Bevan
Journal:  Proc Natl Acad Sci U S A       Date:  1975-01       Impact factor: 11.205

4.  Obesity, longevity, quality of life: alteration by dietary 2-mercaptoethanol.

Authors:  Robert E Click
Journal:  Virulence       Date:  2010-11-01       Impact factor: 5.882

5.  Responses of guinea-pig lymphocytes to mitogens, an antigen, and mixed leucocyte culture in media with and without mercaptoethanol and foetal calf serum.

Authors:  D S Gregerson; B Kelly; J G Levy
Journal:  Immunology       Date:  1975-08       Impact factor: 7.397

6.  In vivo normobaric oxygen exposure depresses spleen cell in vitro Con A response. Effects of 2-mercaptoethanol and peritoneal cells.

Authors:  M A Gougerot-Pocidalo; M Fay; J J Pocidalo
Journal:  Clin Exp Immunol       Date:  1984-11       Impact factor: 4.330

7.  Role of macrophages in the lymphocyte response to Actinomyces viscosus.

Authors:  R M McCarron; J E Fitzgerald; D C Birdsell
Journal:  Infect Immun       Date:  1982-11       Impact factor: 3.441

8.  Long-term cyclophosphamide treatment in rheumatoid patients: effects on serum sulphydryl levels, technetium index, ESR and clinical response.

Authors:  M G Grimaldi
Journal:  Br J Clin Pharmacol       Date:  1981-10       Impact factor: 4.335

9.  Defective monocyte accessory function due to surface sulphydryl (SH) oxidation in rheumatoid arthritis.

Authors:  M J McKeown; N D Hall; J R Corvalan
Journal:  Clin Exp Immunol       Date:  1984-06       Impact factor: 4.330

10.  Immune responses of diabetic animals. Comparison of genetically obese and streptozotocin-diabetic mice.

Authors:  W K Nichols; J B Spellmann; R A Daynes
Journal:  Diabetologia       Date:  1978-05       Impact factor: 10.122
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