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Literature DB >> 4811219

Determination of hepatic 3-hydroxy-3-methylglutaryl CoA reductase activity in man.

G Nicolau, S Shefer, G Salen, E H Mosbach.

Abstract

Procedures were developed for the determination of the activity of the microsomal enzyme 3-hydroxy-3-methylglutaryl CoA reductase (HMG CoA reductase, EC 1.1.1.34) in human liver. The enzyme assay could be carried out with as little as 20 mg of fresh liver tissue, thus making the method applicable to specimens obtained by percutaneous liver biopsy. Experiments were carried out to determine optimal assay conditions and to establish the identity and radiopurity of the reaction product formed from 3-(14)C-labeled 3-hydroxy-3-methylglutaryl CoA. The specific activity of the enzyme was measured in a number of patients with different disorders of lipid metabolism.

Entities: Chemical Gene Species

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Year: 1974 PMID： 4811219

Source DB: PubMed Journal: J Lipid Res ISSN： 0022-2275 Impact factor: 5.922

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Cited

19 in total

1. In vivo regulation of human mononuclear leukocyte 3-hydroxy-3-methylglutaryl coenzyme A reductase. Decreased enzyme catalytic efficiency in familial hypercholesterolemia.

Authors: P W Stacpoole; D M Bridge; I M Alvarez; R B Goldberg; H J Harwood
Journal: J Clin Invest Date: 1987-11 Impact factor: 14.808

Review 2. Bile acid biosynthesis. Pathways and regulation.

Authors: E H Mosbach; G Salen
Journal: Am J Dig Dis Date: 1974-10

3. Biliary cholesterol excretion: a novel mechanism that regulates dietary cholesterol absorption.

Authors: E Sehayek; J G Ono; S Shefer; L B Nguyen; N Wang; A K Batta; G Salen; J D Smith; A R Tall; J L Breslow
Journal: Proc Natl Acad Sci U S A Date: 1998-08-18 Impact factor: 11.205

4. Resistance to chenodeoxycholic acid (CDCA) treatment in obese patients with gall stones.

Authors: J H Iser; P N Maton; G M Murphy; R H Dowling
Journal: Br Med J Date: 1978-06-10

5. Biliary lipid output during three meals and an overnight fast. I. Relationship to bile acid pool size and cholesterol saturation of bile in gallstone and control subjects.

Authors: T C Northfield; A F Hofmann
Journal: Gut Date: 1975-01 Impact factor: 23.059

6. Identification of miR-185 as a regulator of de novo cholesterol biosynthesis and low density lipoprotein uptake.

Authors: Muhua Yang; Weidong Liu; Christina Pellicane; Christine Sahyoun; Biny K Joseph; Christina Gallo-Ebert; Melissa Donigan; Devanshi Pandya; Caroline Giordano; Adam Bata; Joseph T Nickels
Journal: J Lipid Res Date: 2013-12-02 Impact factor: 5.922

Determination of hepatic 3-hydroxy-3-methylglutaryl CoA reductase activity in man.

1. In vivo regulation of human mononuclear leukocyte 3-hydroxy-3-methylglutaryl coenzyme A reductase. Decreased enzyme catalytic efficiency in familial hypercholesterolemia.

Review 2. Bile acid biosynthesis. Pathways and regulation.

3. Biliary cholesterol excretion: a novel mechanism that regulates dietary cholesterol absorption.

4. Resistance to chenodeoxycholic acid (CDCA) treatment in obese patients with gall stones.

5. Biliary lipid output during three meals and an overnight fast. I. Relationship to bile acid pool size and cholesterol saturation of bile in gallstone and control subjects.

6. Identification of miR-185 as a regulator of de novo cholesterol biosynthesis and low density lipoprotein uptake.

7. Rowachol--a possible treatment for cholesterol gallstones.

8. Human cholesterol 7alpha-hydroxylase (CYP7A1) deficiency has a hypercholesterolemic phenotype.

Review 9. Ursodeoxycholic acid: a review of its pharmacological properties and therapeutic efficacy.

10. Hepatic cholesterol and bile acid synthesis in Japanese patients with cholesterol gallstones.