Literature DB >> 4790595

Modified broth-disk method for testing the antibiotic susceptibility of anaerobic bacteria.

T D Wilkins, T Thiel.   

Abstract

The most commonly used method for testing the antibiotic susceptibility of aerobic and facultative bacteria is the disk diffusion method. However, some anaerobic bacteria do not grow well enough in anaerobic jars for performance of disk diffusion tests. A modification of the broth-disk method of Schneierson allowed us to determine antibiotic susceptibility in a completely anaerobic environment. Commercial antibiotic disks were added anaerobically to tubes of prereduced brain heart infusion broth to achieve a concentration of each antibiotic approximating that attainable in blood. The tubes were then inoculated and incubated for 18 h. Resistance or susceptibility to each antibiotic was determined according to the amount of growth in each tube as compared with a control culture without the antibiotic. There was good correlation between results obtained by this broth-disk method and minimal inhibitory concentrations.

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Year:  1973        PMID: 4790595      PMCID: PMC444414          DOI: 10.1128/AAC.3.3.350

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN: 0066-4804            Impact factor:   5.191


  6 in total

1.  [Disk method for determination of sensitivity of anaerobic bacteria to antibiotics].

Authors:  H BEERENS; J GUILLAUME
Journal:  Ann Inst Pasteur (Paris)       Date:  1954-06

2.  Antibiotic sensitivity testing. Report of an international collaborative study.

Authors:  H M Ericsson; J C Sherris
Journal:  Acta Pathol Microbiol Scand B Microbiol Immunol       Date:  1971

3.  Standardized single-disc method for antibiotic susceptibility testing of anaerobic bacteria.

Authors:  T D Wikins; L V Holdeman; I J Abramson; W E Moore
Journal:  Antimicrob Agents Chemother       Date:  1972-06       Impact factor: 5.191

4.  Prototype of a fully automated device for determination of bacterial antibiotic susceptibility in the clinical laboratory.

Authors:  H D Isenberg; A Reichler; D Wiseman
Journal:  Appl Microbiol       Date:  1971-12

5.  The effect of carbon dioxide on the sensitivity of Bacteroides fragilis to certain antibiotics in vitro.

Authors:  H R Ingham; J B Selkon; A A Codd; J H Hale
Journal:  J Clin Pathol       Date:  1970-04       Impact factor: 3.411

6.  Standardized antimicrobial disc susceptibility testing of anaerobic bacteria. I. Susceptibility of Bacteroides fragilis to tetracycline.

Authors:  V L Sutter; Y Y Kwok; S M Finegold
Journal:  Appl Microbiol       Date:  1972-02
  6 in total
  64 in total

1.  Beta-Lactamase activity in strains of Bacteroides melaninogenicus and Bacteroides oralis.

Authors:  A A Salyers; J Wong; T D Wilkins
Journal:  Antimicrob Agents Chemother       Date:  1977-01       Impact factor: 5.191

2.  Evaluation of the modified broth-disk method for determining antibiotic susceptibilities of anaerobic bacteria.

Authors:  D J Blazevic
Journal:  Antimicrob Agents Chemother       Date:  1975-06       Impact factor: 5.191

3.  Stability study of tetracyclines with respect to their use in slow release systems.

Authors:  V C Honnorat-Benabbou; A A Lebugle; B Sallek; D Duffaut-Lagarrigue
Journal:  J Mater Sci Mater Med       Date:  2001-02       Impact factor: 3.896

4.  Isolation of Methanobacterium bryantii from a Deep Aquifer by Using a Novel Broth-Antibiotic Disk Method.

Authors:  E M Godsy
Journal:  Appl Environ Microbiol       Date:  1980-05       Impact factor: 4.792

5.  Simplified method for antimicrobial susceptibility testing of anaerobic bacteria.

Authors:  R J Fass; R B Prior; C A Rotilie
Journal:  Antimicrob Agents Chemother       Date:  1975-10       Impact factor: 5.191

6.  Disk elution method for MICs and MBCs.

Authors:  E Wilson; D A Henry; J A Smith
Journal:  Antimicrob Agents Chemother       Date:  1990-11       Impact factor: 5.191

7.  Diagnosis and therapy of bacterial endophthalmitis, and serum levels of inflammation markers.

Authors:  V Huber-Spitzy; E Arocker-Mettinger; K Herkner; M Schiffbänker; L Georgiew; F J Steinkogler; G Grabner
Journal:  Infection       Date:  1992 May-Jun       Impact factor: 3.553

8.  Susceptibility testing of clinically isolated anaerobic bacteria by an agar dilution technique.

Authors:  W J Brown; P E Waatti
Journal:  Antimicrob Agents Chemother       Date:  1980-04       Impact factor: 5.191

9.  Two-Site Evaluation of the Colistin Broth Disk Elution Test To Determine Colistin In Vitro Activity against Gram-Negative Bacilli.

Authors:  Patricia J Simner; Yehudit Bergman; Marisol Trejo; Ava A Roberts; Remy Marayan; Tsigereda Tekle; Shelley Campeau; Abida Q Kazmi; Drew T Bell; Shawna Lewis; Pranita D Tamma; Romney Humphries; Janet A Hindler
Journal:  J Clin Microbiol       Date:  2019-01-30       Impact factor: 5.948

10.  Collaborative clinical laboratory study of a broth-disk test for determination of bacterial susceptibility to beta-lactams in combination with amdinocillin.

Authors:  H D Isenberg; R C Tilton; A L Barry; G Beskid; R Cleeland; V Fallat; P R Murray; C Pierson; E Squires
Journal:  J Clin Microbiol       Date:  1987-07       Impact factor: 5.948

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