Literature DB >> 478993

Resolution of a gold latensification-elon ascorbic acid developer for Ilford L4 emulsion.

L A Ginsel, J J Onderwater, W T Daems.   

Abstract

The electron-microscopical autoradiographical resolution of a gold latensification-elon ascorbic acid (GEA) developer for Ilford L4 emulsion was determined experimentally, using radioactive line sources of tritiated albumin (Heijnen and Geuze, 1977). For sections with a thickness of 62 nm (SD +/- 11), which were covered with a carbon layer about 5 nm thick and a slightly overlapping monolayer of L4 silver bromide crystals, the measured half-distance (HD) of resolution was 115 nm. This improvement in resolution, the high efficiency of the GEA developer for L4 emulsion (Wisse and Tates, 1968), and the excellent visibility of the cellular structures under the small silver grains, mean that the L4-GEA combination deserves preverence as a method for quantitative electron-microscopical autoradiography.

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Year:  1979        PMID： 478993     DOI： 10.1007/bf00508456

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  8 in total

1.  AUTORADIOGRAPHY WITH THE ELECTRON MICROSCOPE; A QUANTITATIVE EVALUATION.

Authors:  L BACHMANN; M M SALPETER
Journal:  Lab Invest       Date:  1965-06       Impact factor: 5.662

2.  Sensitivity in electron microscope autoradiography. I. The effect of radiation dose.

Authors:  M M Salpeter; M Szabo
Journal:  J Histochem Cytochem       Date:  1972-06       Impact factor: 2.479

3.  Some new aspects of efficiency of electron microscopic autoradiography with tritium.

Authors:  G F Vrensen
Journal:  J Histochem Cytochem       Date:  1970-04       Impact factor: 2.479

4.  Phenidone-ascorbic acid development in electronmicroscopic autoradiography.

Authors:  H Heijnen; H Geuze
Journal:  Histochemistry       Date:  1977-10-03

5.  Resolution in electron microscope radioautography.

Authors:  M M Salpeter; L Bachmann; E E Salpeter
Journal:  J Cell Biol       Date:  1969-04       Impact factor: 10.539

6.  Transport of radiolabelled glycoprotein to cell surface and lysosome-like bodies of absorptive cells in clutured small-intestinal tissue from normal subjects and patients with a lysosomal storage disease.

Authors:  L A Ginsel; J J Onderwater; W T Daems
Journal:  Virchows Arch B Cell Pathol Incl Mol Pathol       Date:  1979-06-29

7.  A comparison of various procedures for fine grain development in electron microscopic radioautography.

Authors:  B Kopriwa
Journal:  Histochemistry       Date:  1975-08-28

8.  AUTORADIOGRAPHY WITH THE ELECTRON MICROSCOPE. A PROCEDURE FOR IMPROVING RESOLUTION, SENSITIVITY, AND CONTRAST.

Authors:  M M SALPETER; L BACHMANN
Journal:  J Cell Biol       Date:  1964-08       Impact factor: 10.539
  8 in total
  7 in total

1.  Presynaptic, high-affinity glutamate uptake in excitatory neuromuscular junctions of the retractor unguis muscle of the locust: determination of the Km value using high resolution autoradiography.

Authors:  J van Marle; T Piek; R D Veldsema-Currie
Journal:  Exp Brain Res       Date:  1986       Impact factor: 1.972

2.  The effect of colchicine on the intracellular transport of 3H-fucose-labelled glycoproteins in the absorptive cells of cultured human small-intestinal tissue. An autoradiographical and biochemical study.

Authors:  J Blok; L A Ginsel; A A Mulder-Stapel; J J Onderwater; W T Daems
Journal:  Cell Tissue Res       Date:  1981       Impact factor: 5.249

3.  The effect of chloroquine on lysosomal function and cell-coat glycoprotein transport in the absorptive cells of cultured human small-intestinal tissue.

Authors:  J Blok; A A Mulder-Stapel; L A Ginsel; W T Daems
Journal:  Cell Tissue Res       Date:  1981       Impact factor: 5.249

4.  Endocytosis in absorptive cells of cultured human small-intestinal tissue: effect of cytochalasin B and D.

Authors:  J Blok; B A Scheven; A A Mulder-Stapel; L A Ginsel; W T Daems
Journal:  Cell Tissue Res       Date:  1982       Impact factor: 5.249

5.  The Origin, Dynamic Morphology, and PI4P-Independent Formation of Encephalomyocarditis Virus Replication Organelles.

Authors:  F J M van Kuppeveld; M Bárcena; C E Melia; H M van der Schaar; A W M de Jong; H R Lyoo; E J Snijder; A J Koster
Journal:  MBio       Date:  2018-04-17       Impact factor: 7.867

6.  Escaping Host Factor PI4KB Inhibition: Enterovirus Genomic RNA Replication in the Absence of Replication Organelles.

Authors:  Charlotte E Melia; Hilde M van der Schaar; Heyrhyoung Lyoo; Ronald W A L Limpens; Qian Feng; Maryam Wahedi; Gijs J Overheul; Ronald P van Rij; Eric J Snijder; Abraham J Koster; Montserrat Bárcena; Frank J M van Kuppeveld
Journal:  Cell Rep       Date:  2017-10-17       Impact factor: 9.423

7.  A unifying structural and functional model of the coronavirus replication organelle: Tracking down RNA synthesis.

Authors:  Eric J Snijder; Ronald W A L Limpens; Adriaan H de Wilde; Anja W M de Jong; Jessika C Zevenhoven-Dobbe; Helena J Maier; Frank F G A Faas; Abraham J Koster; Montserrat Bárcena
Journal:  PLoS Biol       Date:  2020-06-08       Impact factor: 8.029
  7 in total

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