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Literature DB >> 478566

Selection of a chemically defined medium for culturing adult newt forelimb regenerates.

Abstract

Three commercially available tissue-culture media were evaluated for their ability to support continued growth and differentiation of 14-day regenerates of adult newt forelimbs. Serums, embryo extracts, egg ultrafiltrates, and antimicrobial agents were avoided in this analysis. The hormones insulin and L-thyroxine were added to these chemically defined media to enhance continued cellular metabolism and growth. The optimum conditions appeared to be cultivated at 25 degrees C (pH 7.2 to 7.4) in media osmotically adjusted to conditions approximating amphibian blood values (i.e. 225 m0SM for 199, 244 m0SM for CMRL-1066, and 262 m0SM FOR L-15).

Entities: Chemical Species

Mesh：

Substances：
Culture Media

Year: 1979 PMID： 478566 DOI： 10.1007/bf02618408

Source DB: PubMed Journal: In Vitro ISSN： 0073-5655

23 in total

1. The in vitro cultivation of amphibian blastema tissue.

Authors: W J FIMIAN
Journal: J Exp Zool Date: 1959-02

2. Regeneration In Newts In Vitro and In Vivo.

Authors: M LECAMP
Journal: C R Hebd Seances Acad Sci Date: 1948-02-23

3. Stimulation of DNA synthesis and cell division in a chemically defined medium: effect of epidermal growth factor, insulin and vitamin B12 on resting cultures of 3T6 cells.

Authors: K Mierzejewski; E Rozengurt
Journal: Biochem Biophys Res Commun Date: 1976-11-22 Impact factor: 3.575

2. Regulation of Axolotl (Ambystoma mexicanum) Limb Blastema Cell Proliferation by Nerves and BMP2 in Organotypic Slice Culture.

Authors: Jeffrey Lehrberg; David M Gardiner
Journal: PLoS One Date: 2015-04-29 Impact factor: 3.240

2 in total

Selection of a chemically defined medium for culturing adult newt forelimb regenerates.

1. The in vitro cultivation of amphibian blastema tissue.

2. Regeneration In Newts In Vitro and In Vivo.

3. Stimulation of DNA synthesis and cell division in a chemically defined medium: effect of epidermal growth factor, insulin and vitamin B12 on resting cultures of 3T6 cells.

4. Effects of insulin insufficiency on forelimb and tail regeneration in adult Diemictylus viridescens.

5. The relationship between the anterior pituitary gland and the pancreas in tail regeneration of the adult newt.

6. Collagenolytic activity in regenerating forelimbs of the adult newt (Triturus viridescens).

7. Human skin collagenase. Isolation and mechanism of attack on the collagen molecule.

8. Early regenerative responses of amputated limbs of the newt, Notophthalamus viridescens to culture conditions.

9. The urodele limb regeneration blastema: a self-organizing system. i. Differentiation in vitro.

10. A serum macromolecule-supplemented medium for frog cell lines.

1. Stability of positional identity of axolotl blastema cells in vitro.

2. Regulation of Axolotl (Ambystoma mexicanum) Limb Blastema Cell Proliferation by Nerves and BMP2 in Organotypic Slice Culture.