Literature DB >> 477860

[Rat liver S-adenosyl-L-homocysteine hydrolase purification by affinity column chromatography (author's transl)].

B Chabannes, L Cronenberger, H Pachéco.   

Abstract

S-adenosyl-L-homocysteine hydrolase (EC 3.3.1.1) has been purified 240-fold from rat liver by affinity column chromatography on aminohexyl sepharose bound 6-mercaptopurine 9 D-riboside. The purified enzyme was homogeneous by gel electrophoresis.

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Year:  1979        PMID: 477860     DOI: 10.1007/bf01949913

Source DB:  PubMed          Journal:  Experientia        ISSN: 0014-4754


  16 in total

1.  Methods for the analysis and preparation of adenosylmethionine and adenosylhomocysteine.

Authors:  S K Shapiro; D J Ehninger
Journal:  Anal Biochem       Date:  1966-05       Impact factor: 3.365

2.  Methionine metabolism in mammals: synthesis of S-adenosylhomocysteine in rat tissues.

Authors:  J D Finkelstein; B Harris
Journal:  Arch Biochem Biophys       Date:  1973-11       Impact factor: 4.013

3.  In vivo and in vitro metabolism of S-adenosyl-L-homocysteine by Saccharomyces cerevisiae.

Authors:  J A Duerre
Journal:  Arch Biochem Biophys       Date:  1968-03-20       Impact factor: 4.013

4.  Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.

Authors:  P Cuatrecasas
Journal:  J Biol Chem       Date:  1970-06       Impact factor: 5.157

5.  The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors:  K Weber; M Osborn
Journal:  J Biol Chem       Date:  1969-08-25       Impact factor: 5.157

6.  Inhibition of transmethylations of biogenic amines by S-adenosylhomocysteine. Enhancement of transmethylation by adenosylhomocysteinase.

Authors:  T Deguchi; J Barchas
Journal:  J Biol Chem       Date:  1971-05-25       Impact factor: 5.157

7.  Species and tissue differences in the catabolism of S-adenosyl-L-homocysteine: a quantitative, chromatographic study.

Authors:  R A Schatz; C R Vunnam; O Z Sellinger
Journal:  Life Sci       Date:  1977-01-15       Impact factor: 5.037

8.  Metabolism of S-adenosylmethionine in germinating pea seeds: turnover and possible relationships between recycling of sulfur and transmethylation reactions.

Authors:  W A Dodd; E A Cossins
Journal:  Arch Biochem Biophys       Date:  1969-09       Impact factor: 4.013

9.  Mechanism for enzymatic thioether formation. Mechanism of action of S-adenosylhomocysteinase.

Authors:  J L Palmer; R H Abeles
Journal:  J Biol Chem       Date:  1976-09-25       Impact factor: 5.157

10.  Adenosylhomocysteine hydrolase. Crystallization of the purified enzyme and its properties.

Authors:  H H Richards; P K Chiang; G L Cantoni
Journal:  J Biol Chem       Date:  1978-06-25       Impact factor: 5.157

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  2 in total

1.  S-Adenosylhomocysteine hydrolase from human placenta. Affinity purification and characterization.

Authors:  M S Hershfield; V N Aiyar; R Premakumar; W C Small
Journal:  Biochem J       Date:  1985-08-15       Impact factor: 3.857

2.  Affinity-chromatographic purification of S-adenosyl-L-homocysteine hydrolase. Some properties of the enzyme from rat liver.

Authors:  E O Kajander; A M Raina
Journal:  Biochem J       Date:  1981-02-01       Impact factor: 3.857

  2 in total

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