Literature DB >> 476554

Fingerprinting bacterial chromosomal DNA with restriction endonuclease EcoRI: comparison of Rhizobium spp. and identification of mutants.

J R Mielenz, L E Jackson, F O'Gara, K T Shanmugam.   

Abstract

Total cellular DNA from Rhizobium trifolii, R. melitoti, and R. japonicum strains 110 and 117 were prepared. DNA fragments generated with restriction endonuclease EcoRI from these DNA samples were compared in agarose gels after electrophoresis. DNA cleavage patterns generated from R. japonicum strain 110, R. trifolii, and R. meliloti were clearly distinguishable from each other. Restriction endonuclease cleavage patterns of DNA from R. japonicum strain 110 and presumptive R. trifolii mutant strains that nodulate soybean were found to be similar. Rhizobium trifolii mutant strains were also lysed by a phage specific for R. japonicum strain 110. These results show that "R. trifolii mutant strains" are indeed derivatives of R. japonicum strain 110 and not R. trifolii.

Entities:  

Mesh：

Substances：

Year:  1979        PMID： 476554     DOI： 10.1139/m79-118

Source DB:  PubMed          Journal:  Can J Microbiol        ISSN： 0008-4166            Impact factor:   2.419


  11 in total

1.  Genetic Diversity among Rhizobium leguminosarum bv. Trifolii Strains Revealed by Allozyme and Restriction Fragment Length Polymorphism Analyses.

Authors:  D H Demezas; T B Reardon; J M Watson; A H Gibson
Journal:  Appl Environ Microbiol       Date:  1991-12       Impact factor: 4.792

2.  Use of Two-Dimensional Polyacrylamide Electrophoresis to Demonstrate that Putative Rhizobium Cross-Inoculation Mutants Actually Are Contaminants.

Authors:  W T Leps; G P Roberts; W J Brill
Journal:  Appl Environ Microbiol       Date:  1980-02       Impact factor: 4.792

3.  Complementary methodologies to identify specific agrobacterium strains.

Authors:  H Bouzar; L W Moore
Journal:  Appl Environ Microbiol       Date:  1987-11       Impact factor: 4.792

4.  Restriction Endonuclease and nif Homology Patterns of Bradyrhizobium japonicum USDA 110 Derivatives With and Without Nitrogen Fixation Competence.

Authors:  J N Mathis; L D Kuykendall; G H Elkan
Journal:  Appl Environ Microbiol       Date:  1986-03       Impact factor: 4.792

Review 5.  Genome size in bacteria.

Authors:  J T Trevors
Journal:  Antonie Van Leeuwenhoek       Date:  1996-05       Impact factor: 2.271

6.  Analysis of Haemophilus parasuis isolates from southern Ontario swine by restriction endonuclease fingerprinting.

Authors:  N L Smart; O P Miniats; J I MacInnes
Journal:  Can J Vet Res       Date:  1988-07       Impact factor: 1.310

7.  Investigation of a Campylobacter jejuni outbreak by serotyping and chromosomal restriction endonuclease analysis.

Authors:  W C Bradbury; A D Pearson; M A Marko; R V Congi; J L Penner
Journal:  J Clin Microbiol       Date:  1984-03       Impact factor: 5.948

8.  Restriction endonuclease DNA analysis of Leptospira interrogans serovars icterohaemorrhagiae and hebdomadis.

Authors:  R B Marshall; P J Winter; R Yanagawa
Journal:  J Clin Microbiol       Date:  1984-10       Impact factor: 5.948

9.  Bacillus stearothermophilus contains a plasmid-borne gene for alpha-amylase.

Authors:  J R Mielenz
Journal:  Proc Natl Acad Sci U S A       Date:  1983-10       Impact factor: 11.205

10.  Soluble methane monooxygenase component B gene probe for identification of methanotrophs that rapidly degrade trichloroethylene.

Authors:  H C Tsien; R S Hanson
Journal:  Appl Environ Microbiol       Date:  1992-03       Impact factor: 4.792
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.