Literature DB >> 4762392

Purification and properties of an aryl acylamidase of Bacillus sphaericus, catalyzing the hydrolysis of various phenylamide herbicides and fungicides.

G Engelhardt, P R Wallnöfer, R Plapp.   

Abstract

From Bacillus sphaericus ATCC 12123 an aryl acylamidase (EC 3.5.1.13) was purified to homogeneity by ion exchange chromatography, gel filtration, and polyacrylamide gel electrophoresis. The enzyme is inducible by various phenylamides of the acylanilide, phenylcarbamate, and methoxysubstituted phenylurea type. It has a molecular weight of 75,000. Enzyme activity was inhibited by sulfhydryl reagents, several metal ions, and 3,4-dichloroaniline (a product of linuron degradation). A requirement for divalent metal ions in enzyme activity could not be demonstrated. In the presence of 6 M urea an irreversible inactivation of the enzyme occurred. The hydrolysis of L-alanine-4-nitroanilide was competitively inhibited by puromycin.

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Year:  1973        PMID： 4762392      PMCID： PMC379889          DOI： 10.1128/am.26.5.709-718.1973

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


  25 in total

1.  Arylamidase of Neisseria catarrhalis.

Authors:  F J Behal; J D Folds
Journal:  Arch Biochem Biophys       Date:  1967-08       Impact factor: 4.013

2.  Arylamidases of rat liver and kidney.

Authors:  S Mahadevan; A L Tappel
Journal:  J Biol Chem       Date:  1967-05-25       Impact factor: 5.157

3.  [Study of an L-leucyl-beta-naphthylamide hydrolase in relation to sporulation in Bacillus megaterium].

Authors:  J P Aubert; J Millet
Journal:  C R Acad Hebd Seances Acad Sci D       Date:  1965-11-15

4.  Biochemical decomposition of the herbicide N-(3,4-dichlorophenyl)-2-methylpentanamide and related compounds.

Authors:  N E Sharabi; L M Bordeleau
Journal:  Appl Microbiol       Date:  1969-09

5.  A study of human tissue aminopeptidase components.

Authors:  F J Behal; B Asserson; F Dawson; J Hardman
Journal:  Arch Biochem Biophys       Date:  1965-08       Impact factor: 4.013

6.  A comparative study of bacterial alanine aminohydrolases.

Authors:  F J Behal; J D Folds
Journal:  Biochem Biophys Res Commun       Date:  1967-05-05       Impact factor: 3.575

7.  Dipeptidyl arylamidase III of the pituitary. Purification and characterization.

Authors:  S Ellis; J M Nuenke
Journal:  J Biol Chem       Date:  1967-10-25       Impact factor: 5.157

8.  The occurrence of amino acid naphthylamidase in baker's yeast.

Authors:  A Tjeder
Journal:  Acta Chem Scand       Date:  1966

9.  Arylamidase of Neisseria catarrhalis.

Authors:  F J Behal; S T Cox
Journal:  J Bacteriol       Date:  1968-10       Impact factor: 3.490

10.  Estimation of the molecular weights of proteins by Sephadex gel-filtration.

Authors:  P Andrews
Journal:  Biochem J       Date:  1964-05       Impact factor: 3.766
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  14 in total

1.  Aryl acylamidase from Rhodococcus erythropolis NCIB 12273.

Authors:  P A Vaughan; G F Hall; D J Best
Journal:  Appl Microbiol Biotechnol       Date:  1990-10       Impact factor: 4.813

2.  Dynamics of the linuron hydrolase libA gene pool size in response to linuron application and environmental perturbations in agricultural soil and on-farm biopurification systems.

Authors:  Karolien Bers; Kristel Sniegowski; René De Mot; Dirk Springael
Journal:  Appl Environ Microbiol       Date:  2012-02-03       Impact factor: 4.792

3.  A novel hydrolase identified by genomic-proteomic analysis of phenylurea herbicide mineralization by Variovorax sp. strain SRS16.

Authors:  Karolien Bers; Baptiste Leroy; Philip Breugelmans; Pieter Albers; Rob Lavigne; Sebastian R Sørensen; Jens Aamand; René De Mot; Ruddy Wattiez; Dirk Springael
Journal:  Appl Environ Microbiol       Date:  2011-10-14       Impact factor: 4.792

4.  Properties of an immobilized pesticide-hydrolyzing enzyme.

Authors:  D M Munnecke
Journal:  Appl Environ Microbiol       Date:  1977-03       Impact factor: 4.792

5.  A structure-activity study with aryl acylamidases.

Authors:  D T Villarreal; R F Turco; A Konopka
Journal:  Appl Environ Microbiol       Date:  1994-11       Impact factor: 4.792

6.  Amidase activity of some bacteria.

Authors:  A Arnaud; P Galzy; J C Jallageas
Journal:  Folia Microbiol (Praha)       Date:  1976       Impact factor: 2.099

7.  N-acetylanthranilate amidase from Arthrobacter nitroguajacolicus Rü61a, an alpha/beta-hydrolase-fold protein active towards aryl-acylamides and -esters, and properties of its cysteine-deficient variant.

Authors:  Stephan Kolkenbrock; Katja Parschat; Bernd Beermann; Hans-Jürgen Hinz; Susanne Fetzner
Journal:  J Bacteriol       Date:  2006-10-13       Impact factor: 3.490

8.  Enzymatic hydrolysis of organophosphate insecticides, a possible pesticide disposal method.

Authors:  D M Munnecke
Journal:  Appl Environ Microbiol       Date:  1976-07       Impact factor: 4.792

9.  Isolation from agricultural soil and characterization of a Sphingomonas sp. able to mineralize the phenylurea herbicide isoproturon.

Authors:  S R Sørensen; Z Ronen; J Aamand
Journal:  Appl Environ Microbiol       Date:  2001-12       Impact factor: 4.792

10.  Propachlor degradation by a soil bacterial community.

Authors:  D T Villarreal; R F Turco; A Konopka
Journal:  Appl Environ Microbiol       Date:  1991-08       Impact factor: 4.792
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