Literature DB >> 475748

Mass-spectral identification and purification of phycoerythrobilin and phycocyanobilin.

E Fu, L Friedman, H W Siegelman.   

Abstract

The bile-pigment chromophores of C-phycoerythrin (phycoerythrobilin) and C-phycocyanin (phycocyanobilin) were cleaved from their respective proteins with boiling methanol or butan-1-ol. They were purified as dicarboxylic acids by preparative reverse-phase liquid chromatography. Each pigment existed in two principal forms, which were characterized by using 20 pmol samples by proton-transfer chemical-ionization mass spectroscopy. These two principal forms were isomeric species, and all had protonated parent molecular ions with m/e 587, corresponding to a molecular weight of 586.

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Year:  1979        PMID： 475748      PMCID： PMC1186587          DOI： 10.1042/bj1790001

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  5 in total

1.  Mass spectra of ubiquinones and ubiquinols.

Authors:  R F Muraca; J S Whittick; G D Daves; P Friis; K Folkers
Journal:  J Am Chem Soc       Date:  1967-03-15       Impact factor: 15.419

2.  Structure of bilirubin.

Authors:  R Bonnett; J E Davies; M B Hursthouse
Journal:  Nature       Date:  1976-07-22       Impact factor: 49.962

3.  The structure and properties of phycocyanobilin and related bilatrienes.

Authors:  W J Cole; D J Chapman; H W Siegelman
Journal:  Biochemistry       Date:  1968-08       Impact factor: 3.162

4.  Structure of phycocyanobilin.

Authors:  B L Schram; H H Kroes
Journal:  Eur J Biochem       Date:  1971-04-30

5.  Cleavage of phycocyanobilin from C-phycocyanin. Separation and mass spectral identification of the products.

Authors:  R J Beuhler; R C Pierce; L Friedman; H W Siegelman
Journal:  J Biol Chem       Date:  1976-04-25       Impact factor: 5.157
  5 in total
  6 in total

1.  Biosynthesis of cyanobacterial phycobiliproteins in Escherichia coli: chromophorylation efficiency and specificity of all bilin lyases from Synechococcus sp. strain PCC 7002.

Authors:  Avijit Biswas; Yasmin M Vasquez; Tierna M Dragomani; Monica L Kronfel; Shervonda R Williams; Richard M Alvey; Donald A Bryant; Wendy M Schluchter
Journal:  Appl Environ Microbiol       Date:  2010-03-12       Impact factor: 4.792

2.  The native forms of the phycobilin chromophores of algal biliproteins. A clarification.

Authors:  P O'Carra; R F Murphy; S D Killilea
Journal:  Biochem J       Date:  1980-05-01       Impact factor: 3.857

3.  Preparation and properties of crystalline biliverdin IX alpha. Simple methods for preparing isomerically homogeneous biliverdin and [14C[biliverdin by using 2,3-dichloro-5,6-dicyanobenzoquinone.

Authors:  A F McDonagh; L A Palma
Journal:  Biochem J       Date:  1980-08-01       Impact factor: 3.857

4.  Functional analysis of a 450-amino acid N-terminal fragment of phytochrome B in Arabidopsis.

Authors:  Yoshito Oka; Tomonao Matsushita; Nobuyoshi Mochizuki; Tomomi Suzuki; Satoru Tokutomi; Akira Nagatani
Journal:  Plant Cell       Date:  2004-07-23       Impact factor: 11.277

5.  De novo synthetic biliprotein design, assembly and excitation energy transfer.

Authors:  Joshua A Mancini; Molly Sheehan; Goutham Kodali; Brian Y Chow; Donald A Bryant; P Leslie Dutton; Christopher C Moser
Journal:  J R Soc Interface       Date:  2018-04       Impact factor: 4.118

Review 6.  C-Phycocyanin and Phycocyanobilin as Remyelination Therapies for Enhancing Recovery in Multiple Sclerosis and Ischemic Stroke: A Preclinical Perspective.

Authors:  Giselle Pentón-Rol; Javier Marín-Prida; Viviana Falcón-Cama
Journal:  Behav Sci (Basel)       Date:  2018-01-18
  6 in total

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