Literature DB >> 47371

Enzymoimmunoassay of human alpha-fetoprotein.

R Maiolini, B Ferrua, R Masseyeff.   

Abstract

A non-competitive method for the determination of human alpha-fetoprotein (AFP) in serum, using a pure specific antibody linked to glucose oxidase is described. When applied to human AFP, this method gives reproducible results in the range 0.7 to 15 ng/ml, in a relatively short time (6 hr). AFP sera levels of healthy human adults, pregnant women and adults with liver diseases, were tested both by enzymoimmunoassay and radioimmunoassay. In all cases, good agreement was noted between the two methods.

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Year:  1975        PMID: 47371     DOI: 10.1016/0022-1759(75)90006-x

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN: 0022-1759            Impact factor:   2.303


  3 in total

Review 1.  Enzyme immunoassays with special reference to ELISA techniques.

Authors:  A Voller; A Bartlett; D E Bidwell
Journal:  J Clin Pathol       Date:  1978-06       Impact factor: 3.411

2.  Immunolocalization of alpha-fetoprotein in the ovary and hypophysis of immature female rats.

Authors:  D Castelli; C Aussel; M Lafaurie; N Ayraud; C Stora
Journal:  Histochem J       Date:  1982-11

3.  Applications of enzyme-linked immunosorbent assay in veterinary medicine: a bibliography.

Authors:  S Charan; O P Gautam
Journal:  Vet Res Commun       Date:  1984-11       Impact factor: 2.459

  3 in total

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