Literature DB >> 47370

A comparison of the Farr technique with equilibrium dialysis for measurement of antibody concentration and affinity.

Y T Kim, S Kalver, G W Siskind.   

Abstract

The Farr technique has been compared with equilibrium dialysis in regard to its ability to measure antibody concentration and affinity. An excellent qualitative agreement between the two techniques was obtained. Quantitatively, the Farr technique tended to underestimate antibody concentration and overestimate antibody affinity. It is likely that these observations are due to the failure of some low affinity antibodies to bind hapten under the conditions of the Farr assay.

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Year:  1975        PMID: 47370     DOI: 10.1016/0022-1759(75)90005-8

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN: 0022-1759            Impact factor:   2.303


  6 in total

1.  Subunit interactions in mouse myeloma proteins with anti-galactan activity.

Authors:  B N Manjula; C P Glaudemans; E B Mushinski; M Potter
Journal:  Proc Natl Acad Sci U S A       Date:  1976-03       Impact factor: 11.205

2.  Immunochemical properties of oligosaccharide-protein conjugates with Klebsiella-K2 specificity. I. Specificity and crossreactivity of anti-conjugate versus anti-bacterial antibodies.

Authors:  H Geyer; S Stirm; K Himmelspach
Journal:  Med Microbiol Immunol       Date:  1979-01-24       Impact factor: 3.402

3.  Role of T cells in the development of memory B cells. Quantitative and qualitative analysis.

Authors:  D E Lafrenz; T L Feldbush
Journal:  Immunology       Date:  1981-09       Impact factor: 7.397

4.  Binding properties of goat IgM anti-dinitrophenyl antibodies.

Authors:  N Congy; C Mihaesco
Journal:  Immunology       Date:  1978-08       Impact factor: 7.397

5.  Effect of selective ultraviolet phototherapy on DNA and antinuclear antibody titers in psoriatic patients.

Authors:  B Leipold; H Grimm; H J Vogt; W Remy
Journal:  Arch Dermatol Res       Date:  1984       Impact factor: 3.017

6.  Experimental IgA nephropathy.

Authors:  A Rifai; P A Small; P O Teague; E M Ayoub
Journal:  J Exp Med       Date:  1979-11-01       Impact factor: 14.307

  6 in total

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