| Literature DB >> 4723782 |
Abstract
The alkali-labile P content of membrane protein prepared from rapidly frozen rat brain was measured, CuSO(4) being used to inhibit protein phosphatase activity during subcellular fractionation. The P content of the membrane fraction was significantly increased (+12%) over the control value by incubation of homogenates with ATP before fractionation. This suggests that the membrane protein in rat brain is normally only partially phosphorylated.Entities:
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Year: 1973 PMID: 4723782 PMCID: PMC1177709 DOI: 10.1042/bj1330387
Source DB: PubMed Journal: Biochem J ISSN: 0264-6021 Impact factor: 3.857