Literature DB >> 46862

The influence of fixation on staining of glycosaminoglycans in glial cells.

M Lai, I A Lampert, P D Lewis.   

Abstract

Cytoplasmic staining of glial cells by Alcian blue in 0.40 M magnesium chloride can be demonstrated in sections of brain tissue fixed in formol-acetic acid and in Bouin's solution. Phosphate-buffered aldehyde fixatives, whether at neutral or low pH, fail to preserve stainable material.

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Year:  1975        PMID： 46862     DOI： 10.1007/bf00497691

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  8 in total

1.  Differential staining of acid glycosaminoglycans (mucopolysaccharides) by alcian blue in salt solutions.

Authors:  J E Scott; J Dorling
Journal:  Histochemie       Date:  1965-10-01

2.  Demonstration of acidic polyanions in certain glial cells during postnatal rat brain development.

Authors:  I A Lampert; P D Lewis
Journal:  Brain Res       Date:  1974-06-20       Impact factor: 3.252

3.  Histochemistry of Alcian blue. 3. The molecular biological basis of staining by Alcian blue 8GX and analogous phthalocyanins.

Authors:  J E Scott
Journal:  Histochemie       Date:  1972

4.  Distribution and metabolism of mucopolysaccharides and glycoproteins in neuronal perikarya, astrocytes, and oligodendroglia.

Authors:  R U Margolis; R K Margolis
Journal:  Biochemistry       Date:  1974-07-02       Impact factor: 3.162

5.  Histochemical demonstration of acid glycosaminoglycans in the nerve cell cytoplasm of mouse central nervous system.

Authors:  H V Castejón
Journal:  Acta Histochem       Date:  1970       Impact factor: 2.479

6.  Isolation and characterization of glycosaminoglycans in brain of different species.

Authors:  M Singh; E V Chandrasekaran; R Cherian; B K Bachhawat
Journal:  J Neurochem       Date:  1969-07       Impact factor: 5.372

7.  Reversal of protein blocking of basophilia in salt solutions: implications in the localization of polyanions using alcian blue.

Authors:  J E Scott; J Dorling; R A Stockwell
Journal:  J Histochem Cytochem       Date:  1968-05       Impact factor: 2.479

8.  Perfusion-fixation, colchicine and mitotic activity in the adult rat brain.

Authors:  J B Cavanagh; P D Lewis
Journal:  J Anat       Date:  1969-03       Impact factor: 2.610
  8 in total
  5 in total

1.  The use of tannic acid for the ultrastructural visualization of hyaluronic acid.

Authors:  C T Singley; M Solursh
Journal:  Histochemistry       Date:  1980-02

2.  Glycoprotein metabolism in the hypothalamus of rat: significance of glial cells.

Authors:  C Pilgrim; H J Wagner
Journal:  Histochemistry       Date:  1975-12-19

3.  On the Nature of Tintinnid Loricae (Ciliophora: Spirotricha: Tintinnina): a Histochemical, Enzymatic, EDX, and High-resolution TEM Study.

Authors:  Sabine Agatha; Paul Simon
Journal:  Acta Protozool       Date:  2012-06-08       Impact factor: 0.892

4.  The influence of embedding media and fixation on the post-embedment ultrastructural demonstration of complex carbohydrates. II. Dialyzed iron staining.

Authors:  G N Thomopoulos; B A Schulte; S S Spicer
Journal:  Histochemistry       Date:  1983

5.  Epithelial Heparan Sulfate Contributes to Alveolar Barrier Function and Is Shed during Lung Injury.

Authors:  Sarah M Haeger; Xinyue Liu; Xiaorui Han; J Brennan McNeil; Kaori Oshima; Sarah A McMurtry; Yimu Yang; Yilan Ouyang; Fuming Zhang; Eva Nozik-Grayck; Rachel L Zemans; Rubin M Tuder; Julie A Bastarache; Robert J Linhardt; Eric P Schmidt
Journal:  Am J Respir Cell Mol Biol       Date:  2018-09       Impact factor: 7.748
  5 in total

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