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Literature DB >> 46858

Ultrastructural localization of L-alpha-hydroxy acid oxidase in rat liver perioxisomes.

Abstract

The localization of L-alpha-hydroxy acid oxidase in rat liver peroxisomes was studied using slight modifications of the Shnitka and Talibi (1971) method. Best results were obtained with formaldehyde fixation and incubation with glycolate as substrate. Following incubation the copper ferrocyanide reaction product was amplified with 3,3'-diamino-benzidine according to Hanker et al. (1972a,b). Dense reaction product was visible in hepatocyte peroxisomes by light and electron microscopy. Some diffusion of enzyme and/or reaction product into the adjacent cytoplasm occurred around the peroxisomes. Apparent non-specific deposits occurred on the plasmalemma, in the nucleus, and occasionally over mitochondria. Glutaraldehyde fixation severely inhibited enzymatic activity, and the enzyme showed less activity toward L-lactate and DL-alpha-hydroxybutyrate.

Entities: Chemical Disease Species

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Year: 1975 PMID： 46858 DOI： 10.1007/bf00497683

Source DB: PubMed Journal: Histochemistry ISSN： 0301-5564

28 in total

9. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.

Authors: R C Graham; M J Karnovsky
Journal: J Histochem Cytochem Date: 1966-04 Impact factor: 2.479

3. Peroxisomes (microbodies) in the myocardium of rodents and primates. A comparative Ultrastructural cytochemical study.

Authors: L Hicks; H D Fahimi
Journal: Cell Tissue Res Date: 1977-01-04 Impact factor: 5.249

8. Electron microscopic cytochemical localization of alpha-hydroxyacid oxidase in rat liver. Association with the crystalline core and matrix of peroxisomes.

Authors: S Angermüller; C Leupold; A Völkl; H D Fahimi
Journal: Histochemistry Date: 1986

9. The demonstration of arylsulfatases with 4-nitro-1,2-benzenediol mono(hydrogen sulfate) by the formation of osmium blacks at the sites of copper capture.

Authors: J S Hanker; L P Thornburg; P E Yates; D K Romanovicz
Journal: Histochemistry Date: 1975

10. Cytochemical localization of catalase and several hydrogen peroxide-producing oxidases in the nucleoids and matrix of rat liver peroxisomes.

Authors: M Veenhuis; S E Wendelaar Bonga
Journal: Histochem J Date: 1979-09

10 in total

Ultrastructural localization of L-alpha-hydroxy acid oxidase in rat liver perioxisomes.

1. NON-MITOCHONDRIAL OXIDIZING PARTICLES (MICROBODIES) IN RAT LIVER AND KIDNEY AND IN TETRAHYMENA PYRIFORMIS.

2. Cross-linking of bovine pancreatic ribonuclease A with dimethyl adipimidate.

3. Liver peroxisomes, cytology and function.

4. Morphologic and cytochemical identification of peroxisomes in the rat parotid and other exocrine glands.

5. Visualization of peroxisomes (microbodies) and mitochondria with diaminobenzidine.

6. A low-viscosity epoxy resin embedding medium for electron microscopy.

7. Diffusion artifacts in cytochemistry of catalase.

Review 8. Microbodies and related particles. Morphology, biochemistry, and physiology.

9. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.

10. Cytochemical localization of peroxidatic activity of catalase in rat hepatic microbodies (peroxisomes).

1. Cytochemical localization of glycolate dehydrogenase in mitochondria of chlamydomonas.

2. Cytochemical localization of glycolate oxidase in microbodies of Klebsormidium.

3. Peroxisomes (microbodies) in the myocardium of rodents and primates. A comparative Ultrastructural cytochemical study.

4. The cytochemical demonstration of catalase and D-amino acid oxidase in the microbodies of teleost kidney cells.

Review 5. In situ heterogeneity of peroxisomal oxidase activities: an update.

6. Cytochemical demonstration of malate synthase and glycolate oxidase in microbodies of cucumber cotyledons.

7. Determination of unfrozen water in winter cereals at subfreezing temperatures.

8. Electron microscopic cytochemical localization of alpha-hydroxyacid oxidase in rat liver. Association with the crystalline core and matrix of peroxisomes.

9. The demonstration of arylsulfatases with 4-nitro-1,2-benzenediol mono(hydrogen sulfate) by the formation of osmium blacks at the sites of copper capture.

10. Cytochemical localization of catalase and several hydrogen peroxide-producing oxidases in the nucleoids and matrix of rat liver peroxisomes.