On the stimulation of viral DNA polymerase activity by nonionic detergent.

Nonionic detergents stimulate purified RNA-directed DNA polymerase (reverse transcriptase) activity from various RNA tumor viruses ranging from avian to primate species. The stimulatory effect of the nonionic detergent is dependent on the type and amount of template-primer. The greatest stimulation is obtained when high concentrations of (dT)12-18-(rA)n or activated salmon sperm DNA are used as template-primers. Little stimulation is obtained with viral 70S RNA or with (dT)12-18- (dA)n. The detergent stimulation appears to be specific for viral reverse transcriptase since this effect is not observed with purified bacterial DNA polymerase or with three known mammalian cellular DNA polymerases. This finding may, therefore, be a useful additional criterion for distinguishing viral reverse transcriptase isolated from cells from other cellular DNA polymerases. Nonionic detergent also has a stabilizing effect on viral DNA polymerase against thermal inactivation. This stabilizing effect is further enhanced by the presence of template-primer.