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Literature DB >> 4673490

Studies on vaccinia virus-directed deoxyribonucleic acid polymerase.

R V Citarella, R Muller, A Schlabach, A Weissbach.

Abstract

A vaccinia-directed deoxyribonucleic acid (DNA) polymerase has been partially purified from the cytoplasmic fractions of virus-infected HeLa cells. The utilization of natural and synthetic templates by this enzyme resembles that of the host cell DNA-dependent DNA polymerases. The vaccinia DNA polymerase cannot copy ribopolymers or ribonucleic acid but is very effective with an "activated" DNA as template. An exonuclease preferring single-stranded DNA as substrate is found in the most highly purified preparations of the enzyme. The molecular weight of the vaccinia DNA polymerase seems to be about 110,000. The viral DNA polymerase is also found to be associated with purified, infected cell nuclei, and this association may be due, at least in part, to nonspecific adsorption of the vaccinia DNA polymerase by nuclei.

Entities: Disease Gene Species

Mesh：

Substances：

Year: 1972 PMID： 4673490 PMCID： PMC356526

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

14 in total

1. DNA polymerases of tumor virus: specific effect of ethidium bromide on the use of different synthetic templates.

Authors: B Fridlender; A Weissbach
Journal: Proc Natl Acad Sci U S A Date: 1971-12 Impact factor: 11.205

2. Immunological evidence for the appearance of a new DNA-polymerase in cells infected with vaccinia virus.

Authors: W E Magee; O V Miller
Journal: Virology Date: 1967-01 Impact factor: 3.616

3. Purification and properties of deoxyribonucleic acid polymerase from rat liver mitochondria.

Authors: G F Kalf; J J Ch'ih
Journal: J Biol Chem Date: 1968-09-25 Impact factor: 5.157

4. Deoxyribonucleic acid biosynthesis in mitochondria. Purification and general properties of rat liver mitochondrial deoxyribonucleic acid polymerase.

Authors: R R Meyer; M V Simpson
Journal: J Biol Chem Date: 1970-07-10 Impact factor: 5.157

3. Mechanism of inhibition of herpes simplex virus and vaccinia virus DNA polymerases by aphidicolin, a highly specific inhibitor of DNA replication in eucaryotes.

Authors: G Pedrali-Noy; S Spadari
Journal: J Virol Date: 1980-11 Impact factor: 5.103

4. Synthesis of herpes simplex virus, vaccinia virus, and adenovirus DNA in isolated HeLa cell nuclei. I. Effect of viral-specific antisera and phosphonoacetic acid.

Authors: A Bolden; J Aucker; A Weissbach
Journal: J Virol Date: 1975-12 Impact factor: 5.103

5. Transcriptional mapping of the vaccinia virus DNA polymerase gene.

Authors: E V Jones; B Moss
Journal: J Virol Date: 1985-01 Impact factor: 5.103

6. Cauliflower Mosaic Virus replication complexes: characterization of the associated enzymes and of the polarity of the DNA synthesized in vitro.

Authors: P Pfeiffer; P Laquel; T Hohn
Journal: Plant Mol Biol Date: 1984-09 Impact factor: 4.076

7. Effect of aphidicolin on vaccinia virus: isolation of an aphidicolin-resistant mutant.

Authors: F M DeFilippes
Journal: J Virol Date: 1984-11 Impact factor: 5.103

8. Mapping of the vaccinia virus DNA polymerase gene by marker rescue and cell-free translation of selected RNA.

Authors: E V Jones; B Moss
Journal: J Virol Date: 1984-01 Impact factor: 5.103

9. Elucidating and minimizing the loss by recombinant vaccinia virus of human immunodeficiency virus gene expression resulting from spontaneous mutations and positive selection.

Authors: Linda S Wyatt; Patricia L Earl; Wei Xiao; Jeffrey L Americo; Catherine A Cotter; Jennifer Vogt; Bernard Moss
Journal: J Virol Date: 2009-05-06 Impact factor: 5.103

10. Genetic evidence for vaccinia virus-encoded DNA polymerase: isolation of phosphonoacetate-resistant enzyme from the cytoplasm of cells infected with mutant virus.

Authors: B Moss; N Cooper
Journal: J Virol Date: 1982-08 Impact factor: 5.103

Studies on vaccinia virus-directed deoxyribonucleic acid polymerase.

1. DNA polymerases of tumor virus: specific effect of ethidium bromide on the use of different synthetic templates.

2. Immunological evidence for the appearance of a new DNA-polymerase in cells infected with vaccinia virus.

3. Purification and properties of deoxyribonucleic acid polymerase from rat liver mitochondria.

4. Deoxyribonucleic acid biosynthesis in mitochondria. Purification and general properties of rat liver mitochondrial deoxyribonucleic acid polymerase.

5. DNA-dependent DNA polymerases from HeLa cell nuclei. II. Template and substrate utilization.

6. DNA polymerases from human cells.

7. Studies on "early" enzymes in HeLa cells infected with vaccinia virus.

8. Primer requirement and template specificity of the DNA polymerase of RNA tumor viruses.

9. Separation of a new deoxyribonucleic acid polymerase from vaccinia-infected HeLa cells.

10. A simple and rapid method for the isolation of enzymatically active HeLa cell nuclei.

1. Betalactam antibiotics interfere with eukaryotic DNA-replication by inhibiting DNA polymerase alpha.

2. Evidence of a repetitive sequence in vaccinia virus DNA.