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Literature DB >> 465889

A study of the muscarinic receptor by gel electrophoresis.

Abstract

1 Muscarinic receptors from the brain of rat, guinea-pig and frog have been labelled with tritiated propylbenzilylcholine mustard ([3H]-PrBCM). 2 After solubilisation and sodium dodecyl sulphate (SDS)-polycarylamide gel electrophoresis a single radiolabelled peak was seen which was completely suppressed when labelling was carried out in the presence of 10(-6) M atropine. The results suggest the presence of a single polypeptide chain with a molecular weight of similar to or approximately 80,000. 3 In the case of intestinal smooth muscle multiple labelled peaks were found. Precautions against proteolysis led to the identification of a major peak with a molecular weight in the region of 80,000.

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Year: 1979 PMID： 465889 PMCID： PMC2043627 DOI： 10.1111/j.1476-5381.1979.tb13685.x

Source DB: PubMed Journal: Br J Pharmacol ISSN： 0007-1188 Impact factor: 8.739

8 in total

1. Complete purification of the acetylcholine receptor protein from mammalian muscle.

Authors: J O Dolly; E A Barnard
Journal: FEBS Lett Date: 1975-10-01 Impact factor: 4.124

2. Measurement of free electrophoretic mobility and retardation coefficient of protein-sodium dodecyl sulfate complexes by gel electrophoresis. A method to validate molecular weight estimates.

Authors: G A Banker; C W Cotman
Journal: J Biol Chem Date: 1972-09-25 Impact factor: 5.157

3. The properties of muscarinic receptors in mammalian cerebral cortex.

Authors: A S Burgen; C R Hiley; J M Young
Journal: Br J Pharmacol Date: 1974-06 Impact factor: 8.739

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

5. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors: K Weber; M Osborn
Journal: J Biol Chem Date: 1969-08-25 Impact factor: 5.157

6. The binding of antagonists to brain muscarinic receptors.

Authors: E C Hulme; N J Birdsall; A S Burgen; P Mehta
Journal: Mol Pharmacol Date: 1978-09 Impact factor: 4.436

7. The binding of agonists to brain muscarinic receptors.

Authors: N J Birdsall; A S Burgen; E C Hulme
Journal: Mol Pharmacol Date: 1978-09 Impact factor: 4.436

8. Solubilization and polyacrylamide gel electrophoresis of membrane enzymes with detergents.

Authors: B Dewald; J T Dulaney; O Touster
Journal: Methods Enzymol Date: 1974 Impact factor: 1.600

8 in total

13 in total

1. Effect of proteolytic cleavage on functional properties of muscarinic acetylcholine receptors in rat pancreatic and parotid acinar cells.

Authors: S R Hootman; T M Picado-Leonard
Journal: Biochem J Date: 1985-11-01 Impact factor: 3.857

2. Two molecular weight forms of muscarinic acetylcholine receptors in the avian central nervous system: switch in predominant form during differentiation of synapses.

Authors: T H Large; J J Rauh; F G De Mello; W L Klein
Journal: Proc Natl Acad Sci U S A Date: 1985-12 Impact factor: 11.205

3. Monoclonal antibodies detect the conservation of muscarinic cholinergic receptor structure from Drosophila to human brain and detect possible structural homology with alpha 1-adrenergic receptors.

Authors: J C Venter; B Eddy; L M Hall; C M Fraser
Journal: Proc Natl Acad Sci U S A Date: 1984-01 Impact factor: 11.205

4. Molecular characterization of muscarinic receptor subtypes in bovine cerebral cortex by radiation inactivation and molecular exclusion h.p.l.c.

Authors: O Shirakawa; C Tanaka
Journal: Br J Pharmacol Date: 1985-10 Impact factor: 8.739

5. Affinity labeling of muscarinic receptors in rat cerebral cortex with a photolabile antagonist.

Authors: G Amitai; S Avissar; D Balderman; M Sokolovsky
Journal: Proc Natl Acad Sci U S A Date: 1982-01 Impact factor: 11.205

6. Characterization of agonist and antagonist binding to muscarinic cholinergic receptors solubilized from rat cerebral cortex.

Authors: A Adem; M Sabbagh; A Nordberg
Journal: J Neural Transm Date: 1988 Impact factor: 3.575