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Literature DB >> 4651132

Differential inhibition of mammalian ribonucleic acid polymerases by an exotoxin from Bacillus thuringiensis. The direct observation of nucleoplasmic ribonucleic acid polymerase activity in intact nuclei.

Abstract

The effects of the exotoxin from Bacillus thuringiensis on DNA-dependent RNA polymerases from rat liver were examined. The exotoxin inhibits all RNA polymerase activity at both low and high ionic strength in intact nuclei, and soluble enzymes are similarly affected. This inhibition is relieved by ATP. Dephosphorylated exotoxin did not inhibit the soluble enzymes. Nucleolar and nucleoplasmic RNA polymerases respond to different concentration ranges of exotoxin, and the compound can be used in intact nuclei to isolate the nucleoplasmic activity.

Entities: Chemical Species

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Year: 1972 PMID： 4651132 PMCID： PMC1178758 DOI： 10.1042/bj1270619

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

14 in total

1. Separation and characterization of the subunits of ribonucleic acid polymerase.

Authors: R R Burgess
Journal: J Biol Chem Date: 1969-11-25 Impact factor: 5.157

2. Studies with the ribonucleic acid polymerase. II. Kinetic aspects of initiation and polymerization.

Authors: D D Anthony; D A Goldthwait; C W Wu
Journal: Biochemistry Date: 1969-01 Impact factor: 3.162

3. Inhibition of DNA-dependent RNA polymerase by the exotosin of Bacillus thuringiensis var. gelechiae.

Authors: K Sebesta; K Horská
Journal: Biochim Biophys Acta Date: 1968-11-20

4. Isolation of nucleoli in a medium containing spermine and magnesium acetate.

Authors: H Busch; K S Narayan; J Hamilton
Journal: Exp Cell Res Date: 1967-08 Impact factor: 3.905

5. Nuclei from rat liver: isolation method that combines purity with high yield.

Authors: G Blobel; V R Potter
Journal: Science Date: 1966-12-30 Impact factor: 47.728

6. The catabolism of cholesterol in vitro. Formation of 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanoic acid from cholesterol by rat liver.

Authors: D Mendelsohn; L Mendelsohn
Journal: Biochem J Date: 1969-08 Impact factor: 3.857

7. Studies on the pathogenesis of liver necrosis by alpha-amanitin. Effect of alpha-amanitin on ribonucleic acid synthesis and on ribonucleic acid polymerase in mouse liver nuclei.

Authors: F Stirpe; L Fiume
Journal: Biochem J Date: 1967-11 Impact factor: 3.857

8. A purification and some properties of an insecticidal exotoxin from Bacillus thuringiensis Berliner.

Authors: R P Bond; C B Boyce; S J French
Journal: Biochem J Date: 1969-09 Impact factor: 3.857

9. Experimental conditions affecting ribonucleic acid polymerase in isolated rat liver nuclei. Effect of nucleoside triphosphate concentration, temperature, ammonium sulphate and heparin.

Authors: F Novello; F Stirpe
Journal: Biochem J Date: 1969-05 Impact factor: 3.857

10. Inhibition by alpha-amanitin of ribonucleic acid polymerase solubilized from rat liver nuclei.

Authors: F Novello; L Fiume; F Stirpe
Journal: Biochem J Date: 1970-01 Impact factor: 3.857

14 in total

Review 1. The role of ATP-binding cassette transporters in bacterial pathogenicity.

Authors: Victoria G Lewis; Miranda P Ween; Christopher A McDevitt
Journal: Protoplasma Date: 2012-01-13 Impact factor: 3.356

2. Effect of the exotoxin of Bacillus thuringiensis on normal and ecdysone-stimulated ribonucleic acid polymerase activity in intact nuclei from the fat-body of Sarcophaga bullata larvae.

Authors: T J Beebee; R P Bond
Journal: Biochem J Date: 1973-09 Impact factor: 3.857

3. Effect of an exotoxin from Bacillus thuringiensis on deoxyribonucleic acid-dependent ribonucleic acid polymerase in nuclei from adult Sarcophaga bullata. Unusual behaviour of eukaryotic polymerases to inhibitors.

Authors: T J Beebee; R P Bond
Journal: Biochem J Date: 1973-09 Impact factor: 3.857

4. The heat-stable exotoxin of Bacillus thuringiensis.

Authors: J Vănková
Journal: Folia Microbiol (Praha) Date: 1978 Impact factor: 2.099

5. An ABC transporter from Bacillus thuringiensis is essential for beta-exotoxin I production.

Authors: Sylvain Espinasse; Michel Gohar; Didier Lereclus; Vincent Sanchis
Journal: J Bacteriol Date: 2002-11 Impact factor: 3.490

6. Correspondence of high levels of beta-exotoxin I and the presence of cry1B in Bacillus thuringiensis.

Authors: Sylvain Espinasse; Michel Gohar; Josette Chaufaux; Christophe Buisson; Stéphane Perchat; Vincent Sanchis
Journal: Appl Environ Microbiol Date: 2002-09 Impact factor: 4.792

7. Evidence for two immune inhibitors from Bacillus thuringiensis interfering with the humoral defense system of saturniid pupae.

Authors: T Edlund; I Sidén; H G Boman
Journal: Infect Immun Date: 1976-10 Impact factor: 3.441

8. An extracytoplasmic-function sigma factor is involved in a pathway controlling beta-exotoxin I production in Bacillus thuringiensis subsp. thuringiensis strain 407-1.

Authors: Sylvain Espinasse; Michel Gohar; Didier Lereclus; Vincent Sanchis
Journal: J Bacteriol Date: 2004-05 Impact factor: 3.490

9. The effect of an inhibitor of adenylate cyclase on the development of pyrogen, prostaglandin and cyclic AMP fevers in the rabbit.

Authors: G H Willies; C J Woolf; C Rosendorff
Journal: Pflugers Arch Date: 1976-12-28 Impact factor: 3.657

Review 10. Bacillus thuringiensis Is an Environmental Pathogen and Host-Specificity Has Developed as an Adaptation to Human-Generated Ecological Niches.

Authors: Ronaldo Costa Argôlo-Filho; Leandro Lopes Loguercio
Journal: Insects Date: 2013-12-24 Impact factor: 2.769