| Literature DB >> 463 |
P Housset, M Nagy, J Schwencke.
Abstract
A new method is described for the efficient conversion of Schizosaccharomyces pombe cells into protoplasts. The following parameters of guanine uptake determined in whole cells were unchanged in protoplasts: Km value, requirement for an energy source, sensitivity to competitive inhibitors, pH optimum, as well as the typical variation of the initial velocity of uptake observed during the growth phase.Entities:
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Year: 1975 PMID： 463 DOI： 10.1099/00221287-90-2-260
Source DB: PubMed Journal: J Gen Microbiol ISSN： 0022-1287