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Literature DB >> 4628798

Isolation of Vi antigen and a simple method for its measurement.

Abstract

Vi antigen was purified from saline extract of Citrobacter strain 5396/38 by enzymatic digestion, concentration with ethanol, and precipitation with hexadecyltrimethylammonium bromide. The Vi antigen prepared by this method was 18 and 230 times more protective in mice than two other preparations isolated by an earlier method utilizing acid hydrolysis. A sensitive and specific method of electroimmunodiffusion was described for measurement of Vi antigen.

Entities: Chemical Species

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Year: 1972 PMID： 4628798 PMCID： PMC380626 DOI： 10.1128/am.24.4.628-633.1972

Source DB: PubMed Journal: Appl Microbiol ISSN： 0003-6919

17 in total

1. Electrophoretic purification of the Vi antigen.

Authors: F G JARVIS; M T MESENKO; J E KYLE
Journal: J Bacteriol Date: 1960-11 Impact factor: 3.490

2. The VI antigens of the Enterobacteriaceae. I. Purification and chemical properties.

Authors: E E BAKER; R E WHITESIDE; R BASCH; M A DEROW
Journal: J Immunol Date: 1959-12 Impact factor: 5.422

3. Determination of the Vi-antigen content of acetone-dried typhoid vaccines.

Authors: H H COHEN; H A ENGEL
Journal: Antonie Van Leeuwenhoek Date: 1956 Impact factor: 2.271

4. A turbidimetric method for assay of Vi antigen.

Authors: M E WEBSTER; J F SAGIN; M E FREEMAN
Journal: Proc Soc Exp Biol Med Date: 1952-10

5. Antigens of mycobacterial cell walls.

Authors: K H Wong; M J Pickett; S Froman
Journal: Infect Immun Date: 1970-04 Impact factor: 3.441

6. Studies on Vi antigen. II. Purification of Vi antigen from Escherichia coli 5395/38.

Authors: M E WEBSTER; M LANDY; M E FREEMAN
Journal: J Immunol Date: 1952-08 Impact factor: 5.422

Review 7. Typhoid fever: pathogenesis and immunologic control.

Authors: R B Hornick; S E Greisman; T E Woodward; H L DuPont; A T Dawkins; M J Snyder
Journal: N Engl J Med Date: 1970-09-24 Impact factor: 91.245

8. A simple method for the quantitation of submicrogram amounts of bacterial endotoxin.

Authors: R E Pieroni; E J Broderick; A Bundeally; L Levine
Journal: Proc Soc Exp Biol Med Date: 1970-03

9. Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies.

Authors: C B Laurell
Journal: Anal Biochem Date: 1966-04 Impact factor: 3.365

10. SEROLOGICAL RESPONSES OF MAN TO TYPHOID VACCINES.

Authors: A S BENENSON
Journal: Bull World Health Organ Date: 1964 Impact factor: 9.408

19 in total

1. Evaluation in volunteers of a candidate live oral attenuated Salmonella typhi vector vaccine.

Authors: D M Hone; C O Tacket; A M Harris; B Kay; G Losonsky; M M Levine
Journal: J Clin Invest Date: 1992-08 Impact factor: 14.808

Review 2. Vaccines and cell-mediated immunity.

Authors: F M Collins
Journal: Bacteriol Rev Date: 1974-12

3. Diversity of Vi-related antigens in the microcapsule of Salmonella typhi.

Authors: B Szewczyk; A Taylor
Journal: Infect Immun Date: 1980-12 Impact factor: 3.441

4. Modified Vi tests in the screening of typhoid carriers.

Authors: P Y Chau; A C Chan
Journal: J Hyg (Lond) Date: 1976-08

5. Vi antigen from Salmonella typhosa and immunity against typhoid fever. I. Isolation and immunologic properties in animals.

Authors: K H Wong; J C Feeley; R S Northrup; M E Forlines
Journal: Infect Immun Date: 1974-02 Impact factor: 3.441

6. Vi serology in screening of typhoid carriers: improved specificity by detection of Vi antibodies by counterimmunoelectrophoresis.

Authors: P Y Chau; R S Tsang
Journal: J Hyg (Lond) Date: 1982-10

7. Enzyme-linked immunosorbent assay for detection of Salmonella typhi Vi antigen in urine from typhoid patients.

Authors: T J Barrett; J D Snyder; P A Blake; J C Feeley
Journal: J Clin Microbiol Date: 1982-02 Impact factor: 5.948

8. Enzyme-linked immunosorbent assay for detection of human antibodies to Salmonella typhi Vi antigen.

Authors: T J Barrett; P A Blake; S L Brown; K Hoffman; J M Llort; J C Feeley
Journal: J Clin Microbiol Date: 1983-04 Impact factor: 5.948

9. Vi capsular polysaccharide-protein conjugates for prevention of typhoid fever. Preparation, characterization, and immunogenicity in laboratory animals.

Authors: S C Szu; A L Stone; J D Robbins; R Schneerson; J B Robbins
Journal: J Exp Med Date: 1987-11-01 Impact factor: 14.307

10. Evaluation of modified passive haemagglutination assay for Vi antibody estimation in Salmonella typhi infections.

Authors: G Kang; G Sridharan; M V Jesudason; T J John
Journal: J Clin Pathol Date: 1992-08 Impact factor: 3.411