| Literature DB >> 4628098 |
Abstract
An fi(-) type of R factor for transferable chloramphenicol resistance carries the structural gene for a novel species of chloramphenicol acetyltransferase (EC 2.3.1.99). The enzyme associated with the fi(-) plasmid is distinct from that described for fi(+) R factors. The fi(+)- and fi(-)-related variants of chloramphenicol acetyltransferase were hybridized in vivo and in vitro. Both techniques yielded only a single symmetrical heteromeric species rather than the three (A(3)B(1), A(2)B(2), and A(1)B(3)) hybrids predicted from the tetrameric (identical monomers) structure of chloramphenicol acetyltransferase.Entities:
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Year: 1972 PMID： 4628098 PMCID： PMC389704 DOI： 10.1073/pnas.69.10.3049
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205