Literature DB >> 4622818

Purification of Pseudomonas aeruginosa endotoxin by membrane partition chromatography.

N Rubio, R Lopez.   

Abstract

A procedure is described for obtaining large quantities of purified endotoxin of Pseudomonas aeruginosa by using Diaflo ultrafiltration. This method allowed us to isolate from the protein-lipopolysaccharide complex two low-molecular-weight substances which do not play any antigenic role. It provides a useful tool for immunological purposes.

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Year:  1972        PMID: 4622818      PMCID: PMC380320          DOI: 10.1128/am.23.2.211-213.1972

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN: 0003-6919


  8 in total

1.  Decreased rate of synthesis of immunoglobulin (IgG) in rats infected with moloney leukemia virus.

Authors:  S F Cure; N E Cremer
Journal:  J Immunol       Date:  1969-06       Impact factor: 5.422

2.  Isolation of the monomeric subunit of immunoglobulin M with its interchain disulfide bonds intact.

Authors:  J E Morris; F P Inman
Journal:  Biochemistry       Date:  1968-08       Impact factor: 3.162

Review 3.  Molecular aspects of endotoxic reactions.

Authors:  A Nowotny
Journal:  Bacteriol Rev       Date:  1969-03

4.  Action of ethylenediaminetetraacetic acid, tris(hydroxymethyl)-aminomethane, and lysozyme on cell walls of Pseudomonas aeruginosa.

Authors:  S T Cox; R G Eagon
Journal:  Can J Microbiol       Date:  1968-08       Impact factor: 2.419

5.  Characterization of a protein-lipopolysaccharide complex released from cell walls of Pseudomonas aeruginosa by ethylenediaminetetraacetic acid.

Authors:  S W Rogers; H E Gilleland; R G Eagon
Journal:  Can J Microbiol       Date:  1969-07       Impact factor: 2.419

6.  The purification and characterization of L-histidine ammonia-lyse (Pseudomonas).

Authors:  M M Rechler
Journal:  J Biol Chem       Date:  1969-02-25       Impact factor: 5.157

7.  Study of the structure of Shigella flexneri O antigen. I. Chemical degradation.

Authors:  E Hannecart-Pokorni; D Dekegel; F Depuydt; J Dirkx
Journal:  Biochim Biophys Acta       Date:  1970-02-24

8.  Purification of Pseudomonas aeruginosa endotoxin by gel filtration on Sepharose 4B.

Authors:  N Rubio; R Lopez
Journal:  J Chromatogr       Date:  1971-04-22
  8 in total
  1 in total

1.  Immuno-physical properties of Pseudomonas aeruginosa. Protein-lipopolysaccharide complex treated with surfactants.

Authors:  N Rubio; A Portolés; R Lopez
Journal:  Arch Mikrobiol       Date:  1973-12-21
  1 in total

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