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Literature DB >> 4616685

Kinetics of irreversible enzyme inhibition by an unstable inhibitor.

Abstract

A mathematical treatment for the general case of enzyme inactivation by an inhibitor that breaks down in solution in a first-order reaction is presented. Cathepsin D was inactivated by fluorescein isothiocyanate with a K(i) of 4.47mum. Kinetic constants were also determined for the inactivation of cathepsin D by 1,1-bis(diazoacetyl)-2-phenylethane, and the inactivation of pepsin C by diazoacetyl-dl-norleucine methyl ester.

Entities: Chemical Gene

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Year: 1974 PMID： 4616685 PMCID： PMC1168119 DOI： 10.1042/bj1410601

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

11 in total

1. Biosynthesis of the purines. XV. The effect of aza-L-serine and 6-diazo-5-oxo-L-norleucine on inosinic acid biosynthesis de novo.

Authors: B LEVENBERG; I MELNICK; J M BUCHANAN
Journal: J Biol Chem Date: 1957-03 Impact factor: 5.157

2. Antibiotic principle from Moringa pterygosperma. IX. Inhibition of transaminase by isothiocyanates.

Authors: B R DAS; P L NARASIMHA RAO
Journal: Indian J Med Res Date: 1958-01 Impact factor: 2.375

3. Some properties of specific cholinesterase with particular reference to the mechanism of inhibition by diethyl p-nitrophenyl thiophosphate (E 605) and analogues.

Authors: W N ALDRIDGE
Journal: Biochem J Date: 1950-04 Impact factor: 3.857

4. The specificity of cathepsin D.

Authors: J B Ferguson; J R Andrews; I M Voynick; J S Fruton
Journal: J Biol Chem Date: 1973-10-10 Impact factor: 5.157

5. Preparation and properties of lysozyme modified by fluorescein-isothiocyanate.

Authors: M Hiramatsu; N Okabe; K Tomita
Journal: J Biochem Date: 1973-05 Impact factor: 3.387

6. The inhibition of cholinesterase by diethyl phosphorochloridate.

Authors: Y Ashani; P Wins; I B Wilson
Journal: Biochim Biophys Acta Date: 1972-10-12

7. The kinetics of the reaction of N,N-dimethyl-2-phenylaziridinium ion with bovine erythrocyte acetylcholinesterase.

Authors: J E Purdie; R M Heggie
Journal: Can J Biochem Date: 1970-03

8. An active site peptide from pepsin C.

Authors: J Kay; A P Ryle
Journal: Biochem J Date: 1971-06 Impact factor: 3.857

9. Enzyme inhibitors. XVII. Kinetic studies on the irreversible inhibition of adenosine deaminase.

Authors: H J Schaeffer; M A Schwartz; E Odin
Journal: J Med Chem Date: 1967-07 Impact factor: 7.446

10. Bifunctional inhibitors of pepsin.

Authors: S S Husain; J B Ferguson; J S Fruton
Journal: Proc Natl Acad Sci U S A Date: 1971-11 Impact factor: 11.205

10 in total

1. Inactivation of cathepsin D by dithiophosgene and by 2,2-dichloro-1,3-dithiacyclobutanone.

Authors: E T Rakitzis; T B Malliopoulou
Journal: Biochem J Date: 1976-03-01 Impact factor: 3.857

2. Alternative methods for the determination of rate constants describing enzyme inactivation by an unstable inhibitor.

Authors: C M Topham
Journal: Biochem J Date: 1987-09-15 Impact factor: 3.857

3. Kinetics of protein modification, and/or enzyme inactivation, reactions by an unstable modifying agent.

Authors: E T Rakitzis
Journal: Biochem J Date: 1987-09-15 Impact factor: 3.857

4. Computer simulations of the kinetics of irreversible enzyme inhibition by an unstable inhibitor.

Authors: C M Topham
Journal: Biochem J Date: 1986-12-15 Impact factor: 3.857

5. Experimental approach to the kinetic study of unstable site-directed irreversible inhibitors: kinetic origin of the apparent positive co-operativity arising from inactivation of trypsin by p-amidinophenylmethanesulphonyl fluoride.

Authors: J C Espín; J Tudela
Journal: Biochem J Date: 1994-04-01 Impact factor: 3.857