Literature DB >> 4610563

Relaxation complexes of plasmids ColE1 and ColE2: unique site of the nick in the open circular DNA of the relaxed complexes.

M A Lovett, D G Guiney, D R Helinski.   

Abstract

The product of the induced relaxation of supercoiled DNA-protein relaxation complexes of colicinogenic factors E1 (ColE1) and E2 (ColE2) is an open circular DNA molecule with a strand-specific nick. Cleavage of the open circular DNA of each relaxed complex with the EcoRI restriction endonuclease demonstrates that the single-strand break is at a unique position. The site of the single-strand break in the relaxed ColE1 complex is approximately the same distance from the EcoRI cleavage site as the origin of ColE1 DNA replication.

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Year:  1974        PMID： 4610563      PMCID： PMC434282          DOI： 10.1073/pnas.71.10.3854

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  21 in total

1.  Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae.

Authors:  K Danna; D Nathans
Journal:  Proc Natl Acad Sci U S A       Date:  1971-12       Impact factor: 11.205

2.  Strand-specific supercoiled DNA-protein relaxation complexes: comparison of the complexes of bacterial plasmids ColE1 and ColE2.

Authors:  D G Blair; D B Clewell; D J Sheratt; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1971-01       Impact factor: 11.205

3.  Evidence for the existence of the colicinogenic factors E2 and E3 as supercoiled circular DNA-protein relaxation complexes.

Authors:  D B Clewell; D R Helinski
Journal:  Biochem Biophys Res Commun       Date:  1970-08-11       Impact factor: 3.575

4.  Formation of the parental replicative form DNA of bacteriophage phi-X174 and initial events in its replication.

Authors:  B Francke; D S Ray
Journal:  J Mol Biol       Date:  1971-11-14       Impact factor: 5.469

5.  The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophresis. The effects of changes in conformation.

Authors:  U E Loening
Journal:  Biochem J       Date:  1969-06       Impact factor: 3.857

6.  Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors:  D B Clewell; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1969-04       Impact factor: 11.205

7.  A restriction enzyme from Hemophilus influenzae. II.

Authors:  T J Kelly; H O Smith
Journal:  J Mol Biol       Date:  1970-07-28       Impact factor: 5.469

8.  A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.

Authors:  R Radloff; W Bauer; J Vinograd
Journal:  Proc Natl Acad Sci U S A       Date:  1967-05       Impact factor: 11.205

9.  F 1 sex factor of Escherichia coli. Size and purification in the form of a strand-specific relaxation complex of supercoiled deoxyribonucleic acid and protein.

Authors:  B C Kline; D R Helinski
Journal:  Biochemistry       Date:  1971-12-21       Impact factor: 3.162

10.  Structure of replicating simian virus 40 deoxyribonucleic acid molecules.

Authors:  E D Sebring; T J Kelly; M M Thoren; N P Salzman
Journal:  J Virol       Date:  1971-10       Impact factor: 5.103
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  31 in total

Review 1.  Colicinogeny and related phenomena.

Authors:  K G Hardy
Journal:  Bacteriol Rev       Date:  1975-12

2.  Bidirectional replication of plasmid R6K DNA in Escherichia coli; correspondence between origin of replication and position of single-strand break in relaxed complex.

Authors:  M A Lovett; R B Sparks; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1975-08       Impact factor: 11.205

3.  Sequences spanning the EcoRI substrate site.

Authors:  D E Garfin; H W Boyer; H M Goodman
Journal:  Nucleic Acids Res       Date:  1975-10       Impact factor: 16.971

4.  The oriT region of the conjugative transfer system of plasmid pCU1 and specificity between it and the mob region of other N tra plasmids.

Authors:  E S Paterson; V N Iyer
Journal:  J Bacteriol       Date:  1992-01       Impact factor: 3.490

5.  Binding of phosphorylated histone H1 to DNA.

Authors:  R Knippers; B Otto; R Böhme
Journal:  Nucleic Acids Res       Date:  1978-06       Impact factor: 16.971

6.  Structure of the transcriptionally repressed phosphate-repressible acid phosphatase gene (PHO5) of Saccharomyces cerevisiae.

Authors:  L W Bergman; M C Stranathan; L H Preis
Journal:  Mol Cell Biol       Date:  1986-01       Impact factor: 4.272

7.  Molecular characterization of the virD operon from Agrobacterium tumefaciens.

Authors:  S G Porter; M F Yanofsky; E W Nester
Journal:  Nucleic Acids Res       Date:  1987-09-25       Impact factor: 16.971

8.  Insertion element IS102 resides in plasmid pSC101.

Authors:  H Ohtsubo; M Zenilman; E Ohtsubo
Journal:  J Bacteriol       Date:  1980-10       Impact factor: 3.490

9.  Location of two relaxation nick sites in R6K and single sites in pSC101 and RSF1010 close to origins of vegetative replication: implication for conjugal transfer of plasmid deoxyribonucleic acid.

Authors:  A Nordheim; T Hashimoto-Gotoh; K N Timmis
Journal:  J Bacteriol       Date:  1980-12       Impact factor: 3.490

10.  Method for the isolation of the replication region of a bacterial replicon: construction of a mini-F'kn plasmid.

Authors:  M A Lovett; D R Helinski
Journal:  J Bacteriol       Date:  1976-08       Impact factor: 3.490
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