Antibody levels in human sera measured by the fluorescent-antibody technique against the coxsackie B viruses types 1-5 grown in HEp2 cells compared with results obtained by neutralization.

Twenty pairs of acute and convalescent sera were tested for antibody to the coxsackie B viruses types 1-5 by neutralization and fluorescent-antibody techniques. Results by fluorescence were difficult to read and sensitivity was therefore reduced. Rises by neutralization were type specific in the majority of cases, while those by fluorescence were always against more than one viral type.