| Literature DB >> 4605007 |
Abstract
A new radiometric method for the detection of coliform bacteria in water has been described. The method is based on the release of (14)CO(2) from [(14)C]lactose by bacteria suspended in growth medium and incubated at 37 C. The evolved (14)CO(2) is trapped by hyamine hydroxide and counted in a liquid scintillation spectrometer. The method permits the detection of 1 to 10 organisms within 6 h of incubation. Coliform bacteria suspended in water for several days recover from starvation and may be quantitated by the proposed method. Bacteria from water samples may also be concentrated by filtration through membrane filters and detected by the radiometric assay.Entities:
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Year: 1974 PMID: 4605007 PMCID: PMC186680 DOI: 10.1128/am.28.2.169-171.1974
Source DB: PubMed Journal: Appl Microbiol ISSN: 0003-6919