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Literature DB >> 4599623

Studies on in vitro DNA synthesis: purification of dna C gene product containing dna D activity from Escherichia coli.

S Wickner, I Berkower, M Wright, J Hurwitz.

Abstract

The conversion of varphiX174 single-stranded DNA to duplex DNA by extracts of E. coli requires products of the E. coli DNA replication genes. By use of this complementation system, the dna C gene product has been purified from wild-type E. coli as well as from a dna C temperature-sensitive mutant. The latter preparations are temperature sensitive when compared to the wild-type gene product. The dna C and dna D gene products copurify, have similar characteristics, are both temperature sensitive in preparations from dna C temperature-sensitive cells, and are both undetectable in preparations from dna D temperature-sensitive cells.

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Year: 1973 PMID： 4599623 PMCID： PMC433737 DOI： 10.1073/pnas.70.8.2369

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

19 in total

1. Vegetative Replication and Transfer Replication of Deoxyribonucleic Acid in Temperature-Sensitive Mutants of Escherichia coli K-12.

Authors: M G Marinus; E A Adelberg
Journal: J Bacteriol Date: 1970-12 Impact factor: 3.490

2. The characteristics and genetic map location of a temperature sensitive DNA mutant of E. coli K12.

Authors: B Wolf
Journal: Genetics Date: 1972-12 Impact factor: 4.562

3. Isolation and characterization of thermosensitive Escherichia coli mutants defective in deoxyribonucleic acid replication.

Authors: J A Wechsler; V Nüsslein; B Otto; A Klein; F Bonhoeffer; R Herrmann; L Gloger; H Schaller
Journal: J Bacteriol Date: 1973-03 Impact factor: 3.490

4. Isolation and partial characterization of a mutant of Escherichia coli deficient in DNA polymerase II.

Authors: J L Campbell; L Soll; C C Richardson
Journal: Proc Natl Acad Sci U S A Date: 1972-08 Impact factor: 11.205

5. Deoxyribonucleic acid plymerase II. of Escherichia coli. I. The purification and characterization of the enzyme.

Authors: R B Wickner; B Ginsberg; I Berkower; J Hurwitz
Journal: J Biol Chem Date: 1972-01-25 Impact factor: 5.157

6. Function of DNA polymerase 3 in DNA replication.

Authors: V Nüsslein; B Otto; F Bonhoeffer; H Schaller
Journal: Nat New Biol Date: 1971-12-29

7. Escherichia coli mutants temperature-sensitive for DNA synthesis.

Authors: J A Wechsler; J D Gross
Journal: Mol Gen Genet Date: 1971

8. RNA synthesis initiates in vitro conversion of M13 DNA to its replicative form.

Authors: W Wickner; D Brutlag; R Schekman; A Kornberg
Journal: Proc Natl Acad Sci U S A Date: 1972-04 Impact factor: 11.205

9. Conversion of phiX174 and fd single-stranded DNA to replicative forms in extracts of Escherichia coli.

Authors: R B Wickner; M Wright; S Wickner; J Hurwitz
Journal: Proc Natl Acad Sci U S A Date: 1972-11 Impact factor: 11.205

10. A DNA-unwinding protein isolated from Escherichia coli: its interaction with DNA and with DNA polymerases.

Authors: N Sigal; H Delius; T Kornberg; M L Gefter; B Alberts
Journal: Proc Natl Acad Sci U S A Date: 1972-12 Impact factor: 11.205

16 in total

1. Genetic and phenotypic characterization of dnaC mutations.

Authors: J A Wechsler
Journal: J Bacteriol Date: 1975-02 Impact factor: 3.490

2. Association of phiX174 DNA-dependent ATPase activity with an Escherichia coli protein, replication factor Y, required for in vitro synthesis of phiX174 DNA.

Authors: S Wickner; J Hurwitz
Journal: Proc Natl Acad Sci U S A Date: 1975-09 Impact factor: 11.205

3. Stimulation of DNA polymerase by factors isolated from Novikoff hepatoma.

Authors: G S Probst; D M Stalker; D W Mosbaugh; R R Meyer
Journal: Proc Natl Acad Sci U S A Date: 1975-03 Impact factor: 11.205

Review 4. Recalibrated linkage map of Escherichia coli K-12.

Authors: B J Bachmann; K B Low; A L Taylor
Journal: Bacteriol Rev Date: 1976-03

5. Replication of small plasmids in extracts of Escherichia coli: involvement of the dnaB and dnaC protein in the replication of early replicative intermediates.

Authors: W L Staudenbauer; E Lanka; H Schuster
Journal: Mol Gen Genet Date: 1978-07-04