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Literature DB >> 4596383

Tryptic soy bile-kanamycin test for the indentification of Bacteroides fragilis.

Abstract

A simple and practical test for the identification of Bacteroides fragilis is described. It utilizes two well-known properties of this species, i.e., stimulation of growth by bile and resistance to kanamycin. The test media are a tryptic-soy bile agar plate and a supplemented blood agar plate on which a kanamycin 1,000-mug/ml disk is placed. Incubation is for 24 h at 37 C in GasPak. The results of screening 190 strains, mostly clinical isolates, indicate that B. fragilis can be easily and reliably distinguished from other Bacteroides and from Fusobacterium species by its growth on tryptic-soy bile agar and resistance to kanamycin.

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Year: 1974 PMID： 4596383 PMCID： PMC380069 DOI： 10.1128/am.27.3.480-483.1974

Source DB: PubMed Journal: Appl Microbiol ISSN： 0003-6919

12 in total

Tryptic soy bile-kanamycin test for the indentification of Bacteroides fragilis.

1. Comparison of three blood culture media for recovery of anaerobic bacteria.

2. Isolation of anaerobic bacteria from clinical specimens.

3. Anaerobic bacterial infections of the female genital tract.

4. "Bacteroides" bacteremia.

Review 5. Anaerobic bacteria of the gastrointestinal flora and their occurrence in clinical infections.

6. Antibiotic disc susceptibility tests for rapid presumptive identification of Gram-negative anaerobic bacilli.

7. Practical method for isolation of anerobic bacteria in the clinical laboratory.

8. Antibiotic susceptibility patterns as aids in classification and characterization of gram-negative anaerobic bacilli.

9. Effect of bile and desoxycholate on gram-negative anaerobic bacteria.

10. Bacteriostatic and bactericidal activities of various antibiotics against Bacteroides fragilis.

1. Evaluation of simplified dichotomous schemata for the identification of anaerobic bacteria from clinical material.

2. Lysis of halobacteria in bacto-peptone by bile acids.

3. Evaluation of kanamycin-esculin bile agar for isolation and presumptive identification of Bacteroides fragilis group.

4. Effect of selective media on recovery of obligately anaerobic gram-negative rods from human faeces.

5. Purification and characterization of a bacteriocin from Bacteroides fragilis.

6. Rapid identification of Bacteroides fragilis with bile and antibiotic disks.

7. New medium for selection and presumptive identification of the Bacteroides fragilis group.

8. Isolation of Bacteroides fragilis from the feces of diarrheic calves and lambs.

9. Nutritional features of Bacteroides fragilis subsp. fragilis.

10. Evaluation of the sodium polyanethol sulfonate disk test for the identification of Peptostreptococcus anaerobius.