Literature DB >> 45927

Glycocalyx of epidermal cells in vitro: demonstration and enzymatic removal.

P Fritsch, K Wolff, H Hönigsmann.   

Abstract

Guinea-pig epidermal cells in culture possess a glycocalyx coat similar to that in vivo, as revealed by the ruthenium red stating technique. Trypsin, phospholipase C, and lysozyme do not produce any changes of the glycocalyx, while hyaluronidase and neuraminidase lead to partial and subcomplete removal respectively. Cells stripped of their glycocalyx coat by neuraminidase do not detach from the support and do not show any signs of toxicity. There is complete reconstitution of the glycocalyx within 24 hr.

Entities:  

Mesh：

Substances：

Year:  1975        PMID： 45927     DOI： 10.1111/1523-1747.ep12540891

Source DB:  PubMed          Journal:  J Invest Dermatol        ISSN： 0022-202X            Impact factor:   8.551


  6 in total

1.  Long-term tissue culture of epithelial-like cells from human skin (NCTC strain 2544). I. Measurement of viscosity.

Authors:  A Neufahrt; F J Förster; H Heine; G Schaeg; G Leonhardi
Journal:  Arch Dermatol Res       Date:  1976-10-27       Impact factor: 3.017

2.  Ultrastructural demonstration of cell coat on the cell surfaces of normal human oesophageal epithelium.

Authors:  K R Logan; D Hopwood; G Milne
Journal:  Histochem J       Date:  1977-07

3.  Immunochemical localization of in vivo bound immunoglobulins in pemphigus vulgaris epidermis. Employment of a peroxidase-antiperoxidase multistep technique for light and electron microscopy.

Authors:  H Hönigsmann; K Holubar; K Wolff; E H Beutner
Journal:  Arch Dermatol Res       Date:  1975-12-10       Impact factor: 3.017

4.  Ultrastructural studies on the external surface coat of epidermal cells: alcian blue-lanthanum nitrate staining during wound healing.

Authors:  W S Krawczyk
Journal:  Arch Dermatol Res       Date:  1976-04-21       Impact factor: 3.017

5.  Histochemistry of the intercellular substance of the normal and psoriatic human epidermis.

Authors:  D Cerimele; C Del Forno; F Serri
Journal:  Arch Dermatol Res       Date:  1978-06-29       Impact factor: 3.017

6.  Long-term tissue culture of epithelial-like cells from human skin (NCTC strain 2544). II. Viscosity changes after enzyme treatment.

Authors:  A Neufahrt; F J Förster; K Gottschalk; G Leonhardi
Journal:  Arch Dermatol Res       Date:  1978-02-15       Impact factor: 3.017
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.