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Literature DB >> 4591478

Axenic culture of myxamoebae of the myxomycete Physarum polycephalum.

Abstract

Myxamoebae of the acellular slime mold Physarum polycephalum have been cultured axenically in a soluble medium. The growth medium contains bovine serum albumin, embryo extracts, liver infusion broth, peptone, and glucose. Cell densities ranging from 3 x 10(6) to 5 x 10(6) cells/ml have been obtained with this medium. To date, myxamoebae have been serially transferred more than 100 times without deleterious effect.

Entities: Chemical Species

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Year: 1972 PMID： 4591478 PMCID： PMC251263 DOI： 10.1128/jb.111.1.242-247.1972

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

8 in total

1. Nutrition of cellular slime molds. II. Growth of Polysphondylium pallidum in axenic culture.

Authors: H R HOHL; K B RAPER
Journal: J Bacteriol Date: 1963-01 Impact factor: 3.490

2. The pure culture of Physarum polycephalum on a partially defined soluble medium.

Authors: J W DANIEL; H P RUSCH
Journal: J Gen Microbiol Date: 1961-05

3. Nutritional and related biological studies on the free-living soil amoeba, Hartmannella rhysodes.

Authors: R N BAND
Journal: J Gen Microbiol Date: 1959-08

4. A serum-free nutrient solution sustaining rapid and continuous proliferation of strain L (Earle) mouse cells.

Authors: C WAYMOUTH
Journal: J Natl Cancer Inst Date: 1956-09 Impact factor: 13.506

5. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

6. ORGANIC REQUIREMENTS AND SYNTHETIC MEDIA FOR GROWTH OF THE MYXOMYCETE PHYSARUM POLYCEPHALUM.

Authors: J W DANIEL; K L BABCOCK; A H SIEVERT; H P RUSCH
Journal: J Bacteriol Date: 1963-08 Impact factor: 3.490

7. Hematin--requiring plasmodial myxomycete.

Authors: J W DANIEL; J KELLEY; H P RUSCH
Journal: J Bacteriol Date: 1962-11 Impact factor: 3.490

8. NUTRITION OF CELLULAR SLIME MOLDS. III. SPECIFIC GROWTH REQUIREMENTS OF POLYSPHONDYLIUM PALLIDUM.

Authors: H HOHL; K B RAPER
Journal: J Bacteriol Date: 1963-12 Impact factor: 3.490

8 in total

5 in total

Axenic culture of myxamoebae of the myxomycete Physarum polycephalum.

1. Nutrition of cellular slime molds. II. Growth of Polysphondylium pallidum in axenic culture.

2. The pure culture of Physarum polycephalum on a partially defined soluble medium.

3. Nutritional and related biological studies on the free-living soil amoeba, Hartmannella rhysodes.

4. A serum-free nutrient solution sustaining rapid and continuous proliferation of strain L (Earle) mouse cells.

5. Protein measurement with the Folin phenol reagent.

6. ORGANIC REQUIREMENTS AND SYNTHETIC MEDIA FOR GROWTH OF THE MYXOMYCETE PHYSARUM POLYCEPHALUM.

7. Hematin--requiring plasmodial myxomycete.

8. NUTRITION OF CELLULAR SLIME MOLDS. III. SPECIFIC GROWTH REQUIREMENTS OF POLYSPHONDYLIUM PALLIDUM.

1. Growth of the haploid phase of the myxomycete Physarum flavicomum in defined minimal medium.

2. A method of indirect mutant selection in Physarum polycephalum using the antibiotic netropsin.

3. Viability of Physarum polycephalum spores and ploidy of plasmodial nuclei.

4. Effect of microtubule-disrupting drugs on protein and RNA synthesis in Physarum polycephalum amoebae.

5. Cycloheximide resistance of Physarum polycephalum.