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Literature DB >> 4590174

Immunological functions of lymphocytes fractionated with antigen-derivatized fibers.

U Rutishauser, P D'Eustachio, G M Edelman.

Abstract

Specific antigen-binding cells from spleens of immune and nonimmune mice were isolated by the method of fiber fractionation. After removal from the fibers, these cells were assayed for their viability, their ability to rebind to fibers of the same specificity, and their in vivo response to the antigen after transfer to syngeneic irradiated recipients. These experiments indicate that the fiber method yields highly enriched populations of specific antigen-binding cells that are viable and include antigen-sensitive bone marrow-derived cells capable of undergoing mitosis and differentiating into antibody-secreting cells.

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Year: 1973 PMID： 4590174 PMCID： PMC427352 DOI： 10.1073/pnas.70.12.3894

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

10 in total

1. Restriction of the mobility of lymphocyte immunoglobulin receptors by concanavalin A.

Authors: I Yahara; G M Edelman
Journal: Proc Natl Acad Sci U S A Date: 1972-03 Impact factor: 11.205

2. Binding of thymus- and bone marrow-derived lymphoid cells to antigen-derivatized fibers.

Authors: U Rutishauser; G M Edelman
Journal: Proc Natl Acad Sci U S A Date: 1972-12 Impact factor: 11.205

3. Demonstration that antigen-binding cells are precursors of antibody-producing cells after purification with a fluorescence-activated cell sorter.

Authors: M H Julius; T Masuda; L A Herzenberg
Journal: Proc Natl Acad Sci U S A Date: 1972-07 Impact factor: 11.205

4. Cell separation on affinity columns: the isolation of immunospecific precursor cells from unimmunized mice (lactoside hapten-lymphocyte receptors-immunology).

Authors: C Henry; J Kimura; L Wofsy
Journal: Proc Natl Acad Sci U S A Date: 1972-01 Impact factor: 11.205

5. Enrichment of memory cells carrying receptors for a protein antigen (HSA).

Authors: D Sulitzeanu; M Axelrad
Journal: Immunology Date: 1973-05 Impact factor: 7.397

6. Antitrinitrophenyl (TNP) plaque assay. Primary response of Balb/c mice to soluble and particulate immunogen.

Authors: M B Rittenberg; K L Pratt
Journal: Proc Soc Exp Biol Med Date: 1969-11

7. Cell fractionation and arrangement on fibers, beads, and surfaces.

Authors: G M Edelman; U Rutishauser; C F Millette
Journal: Proc Natl Acad Sci U S A Date: 1971-09 Impact factor: 11.205

8. Specific fractionation of immune cell populations.

Authors: U Rutishauser; C F Millette; G M Edelman
Journal: Proc Natl Acad Sci U S A Date: 1972-06 Impact factor: 11.205

9. Cell separation on antigen-coated columns. Elimination of high rate antibody-forming cells and immunological memory cells.

Authors: H Wigzell; B Andersson
Journal: J Exp Med Date: 1969-01-01 Impact factor: 14.307

10. A new method for the enumeration of antigen-reactive cells responsive to a purified protein antigen.

Authors: W D Armstrong; E Diener
Journal: J Exp Med Date: 1969-02-01 Impact factor: 14.307

10 in total

9 in total

1. Enrichment of memory cells carrying receptors for a protein antigen (HSA). II. Improved enrichment technique, using BSA density gradient separation.

Authors: R Tanenbaum; D Sulitzeanu
Journal: Immunology Date: 1975-09 Impact factor: 7.397

9. Isolation of antigen-binding cells from unprimed mice: demonstration of antibody-forming cell precursor activity and correlation between precursor and secreted antibody avidities.

Authors: M H Julius; L A Herzenberg
Journal: J Exp Med Date: 1974-10-01 Impact factor: 14.307

9 in total

Immunological functions of lymphocytes fractionated with antigen-derivatized fibers.

1. Restriction of the mobility of lymphocyte immunoglobulin receptors by concanavalin A.

2. Binding of thymus- and bone marrow-derived lymphoid cells to antigen-derivatized fibers.

3. Demonstration that antigen-binding cells are precursors of antibody-producing cells after purification with a fluorescence-activated cell sorter.

4. Cell separation on affinity columns: the isolation of immunospecific precursor cells from unimmunized mice (lactoside hapten-lymphocyte receptors-immunology).

5. Enrichment of memory cells carrying receptors for a protein antigen (HSA).

6. Antitrinitrophenyl (TNP) plaque assay. Primary response of Balb/c mice to soluble and particulate immunogen.

7. Cell fractionation and arrangement on fibers, beads, and surfaces.

8. Specific fractionation of immune cell populations.

9. Cell separation on antigen-coated columns. Elimination of high rate antibody-forming cells and immunological memory cells.

10. A new method for the enumeration of antigen-reactive cells responsive to a purified protein antigen.

1. Enrichment of memory cells carrying receptors for a protein antigen (HSA). II. Improved enrichment technique, using BSA density gradient separation.

2. Morphology, motility, and surface behavior of lymphocytes bound to nylon fibers.

3. Single cell studies on the antibody-forming potential of fractionated, hapten-specific B lymphocytes.

4. Separation by column chromatography of cells active in delayed-onset hypersensitivities.

5. Receptor mobility and the mechanism of cell-cell binding induced by concanavalin A.

6. Frequency and avidity of specific antigen-binding cells in developing mice.

7. Cell-to-cell binding induced by different lectins.

8. Separation of antigen-specific lymphocytes. II. Enrichment of hapten-specific antibody-forming cell precursors.

9. Isolation of antigen-binding cells from unprimed mice: demonstration of antibody-forming cell precursor activity and correlation between precursor and secreted antibody avidities.