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Literature DB >> 4589891

Asymmetry in the 50S ribosomal subunit of Escherichia coli.

P B Moore, D M Engelman, B P Schoenborn.

Abstract

A substantial separation of the centers of mass of the protein and RNA portions of the 50S ribosomal subunit has been achieved using neutron scattering. This separation reveals that the subunit has a protein-rich side, a finding which is inconsistent with many models proposed for the structure. By analyzing the variation of the radius of gyration of the subunit under conditions in which the contributions of the protein and RNA were separately enhanced by deuteration and comparing these radii to the undeuterated subunit radius, we have obtained the following values. The radius of gyration of the whole particle is 78.0 +/- 0.95 A, that of the RNA moiety is 72.5 +/- 1.5 A, and that of the protein is 73.4 +/- 2.0 A. These data give a separation of the centers of mass of the RNA and protein distributions of 57.7 +/- 10 A.

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Year: 1974 PMID： 4589891 PMCID： PMC387959 DOI： 10.1073/pnas.71.1.172

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

28 in total

1. Inhibition by thiostrepton of the formation of a ribosome-bound guanine nucleotide complex.

Authors: B Weisblum; V Demohn
Journal: FEBS Lett Date: 1970-12 Impact factor: 4.124

2. Inability of E. coli ribosomes to interact simultaneously with the bacterial elongation factors EF Tu and EF G.

Authors: D Richter
Journal: Biochem Biophys Res Commun Date: 1972-03-10 Impact factor: 3.575

3. A molecular weight determination of the 16S ribosomal ribonucleic acid from Escherichia coli.

Authors: J P Ortega; W E Hill
Journal: Biochemistry Date: 1973-08-14 Impact factor: 3.162

4. Reaction of N-ethyl maleimide with the ribosomes of Escherichia coli.

Authors: P B Moore
Journal: J Mol Biol Date: 1971-08-28 Impact factor: 5.469

5. On the catalytic center of peptidyl transfer: a part of the 50 S ribosome structure.

Authors: T Staehelin; D M Maglott; R E Monro
Journal: Cold Spring Harb Symp Quant Biol Date: 1969

6. Interaction of E. coli G factor with the 50S ribosomal subunit.

Authors: J W Bodley; L Lin
Journal: Nature Date: 1970-07-04 Impact factor: 49.962

7. X-ray scattering study of ribosomes from Escherichia coli.

Authors: W E Hill; J D Thompson; J W Anderegg
Journal: J Mol Biol Date: 1969-08-28 Impact factor: 5.469

8. Inhibition by thiopeptin of ribosomal functions associated with T and G factors.

Authors: T Kinoshita; Y Liou; N Tanaka
Journal: Biochem Biophys Res Commun Date: 1971-08-20 Impact factor: 3.575

9. Protection of ribosomes from thiostrepton inactivation by the binding of G factor and guanosine diphosphate.

Authors: J H Highland; L Lin; J W Bodley
Journal: Biochemistry Date: 1971-11-23 Impact factor: 3.162

10. Inhibition by siomycin and thiostrepton of both aminoacyl-tRNA and factor G binding to ribosomes.

Authors: J Modolell; B Cabrer; A Parmeggiani; D Vazquez
Journal: Proc Natl Acad Sci U S A Date: 1971-08 Impact factor: 11.205

6 in total

1. Low-angle neutron scattering from chromatin subunit particles.

Authors: J F Pardon; D L Worcester; J C Wooley; K Tatchell; K E Van Holde; B M Richards
Journal: Nucleic Acids Res Date: 1975-11 Impact factor: 16.971

2. Contrast variation study of specifically deuterated Escherichia coli ribosomal subunits.

Authors: R R Crichton; D M Engleman; J Haas; M H Koch; P B Moore; R Parfait; H B Stuhrmann
Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

3. Neutron scattering measurements of separation and shape of proteins in 30S ribosomal subunit of Escherichia coli: S2-S5, S5-S8, S3-S7.

Authors: D M Engelman; P B Moore; B P Schoenborn
Journal: Proc Natl Acad Sci U S A Date: 1975-10 Impact factor: 11.205