Transduction of Gal+ by coliphage T1. 3. Requirement for transcription and translation in recipient cells.

A 10- to 15-min derepression of a lambda prophage in a Gal(-) recipient during early infection with a transducing lysate of coliphage T1am will cause an increase in the efficiency of transduction of Gal(+). An increase in the efficiency of transduction occurs when the donor is either nonlysogenic or lysogenic for lambda; the increase is blocked by rifampin or chloramphenicol. With strain R901 it has been shown that efficient transduction can be blocked by treatment with rifampin after all chloramphenicol-sensitive steps have occurred.