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Literature DB >> 4583239

Isolation of temperature-sensitive membrane mutants of Escherichia coli K-12.

J F Steenbakkers, J H Broekman, A Kerkenaar, P G de Haan.

Abstract

Mutants with impaired biosynthesis of unsaturated fatty acids or altered metabolism of the phospholipids were isolated at a rather high frequency from a set of temperature-sensitive lysis mutants. It is suggested that preselection for the lysis phenotype makes it possible to isolate several kinds of mutants affected in the integrity of the cytoplasmic membrane.

Entities: Chemical Species

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Year: 1973 PMID： 4583239 PMCID： PMC285415 DOI： 10.1128/jb.116.2.535-540.1973

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

15 in total

Review 1. Metabolism and function of the membrane phospholipids of Escherichia coli.

Authors: J E Cronan; P R Vagelos
Journal: Biochim Biophys Acta Date: 1972-02-14

2. Temperature-sensitive mutants of Escherichia coli K-12 with low activities of the L-alanine adding enzyme and the D-alanyl-D-alanine adding enzyme.

Authors: E J Lugtenberg; A v Schijndel-van Dam
Journal: J Bacteriol Date: 1972-04 Impact factor: 3.490

3. Temperature-sensitive mutants of Escherichia coli K-12 with low activity of the diaminopimelic acid adding enzyme.

Authors: E J Lugtenberg; A v Schijndel-van Dam
Journal: J Bacteriol Date: 1972-04 Impact factor: 3.490

4. Isolation and characterization of a temperature-sensitive mutant of Escherichia coli with a lesion in the acylation of lysophosphatidic acid.

Authors: K Hechemy; H Goldfine
Journal: Biochem Biophys Res Commun Date: 1971-01-22 Impact factor: 3.575

Isolation of temperature-sensitive membrane mutants of Escherichia coli K-12.

Review 1. Metabolism and function of the membrane phospholipids of Escherichia coli.

2. Temperature-sensitive mutants of Escherichia coli K-12 with low activities of the L-alanine adding enzyme and the D-alanyl-D-alanine adding enzyme.

3. Temperature-sensitive mutants of Escherichia coli K-12 with low activity of the diaminopimelic acid adding enzyme.

4. Isolation and characterization of a temperature-sensitive mutant of Escherichia coli with a lesion in the acylation of lysophosphatidic acid.

5. A mutant of Escherichia coli defective in phosphatidic acid synthesis.

6. EFFECT OF TEMPERATURE ON THE COMPOSITION OF FATTY ACIDS IN ESCHERICHIA COLI.

7. Lipids of Salmonella typhimurium and Escherichia coli: structure and metabolism.

8. Effects of oleate starvation in a fatty acid auxotroph of Escherichia coli K-12.

9. The effect of exogenous fatty acids on fatty acid metabolism in Escherichia coli K-12.

10. Induction of the lactose transport system in a lipid-synthesis-defective mutant of Escherichia coli.

1. Altered phospholipid metabolism in a temperature-sensitive mutant of a thermophilic bacillus.

2. Metabolism of glycerol-3-phosphate and phospholipids in a temperature-sensitive lysis mutant of Escherichia coli.

3. Bacillus subtilis mutant with temperature-sensitive net synthesis of phosphatidylethanolamine.