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Literature DB >> 4583027

In vivo splicing of protein: one continuous polypeptide from two independently functioning operons.

Abstract

Active-beta-galactosidase (EC 3.2.1.23) is often formed in rec(-) merozygotes containing a pair of mutations in the z gene of the lac operon. Contrary to expectation, with certain pairs of mutants this enzyme, upon dissociation, does not yield two independent polypeptides but only a single continuous protomer. Both genetic recombination and suppression have been ruled out as the source of this phenomenon. Therefore we believe we are observing the synthesis of one protein from two independently functioning genes.

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Year: 1973 PMID： 4583027 PMCID： PMC427149 DOI： 10.1073/pnas.70.10.2969

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

24 in total

1. On the determination of molecular weight of proteins and protein subunits in the presence of 6 M guanidine hydrochloride.

Authors: A Ullmann; M E Goldberg; D Perrin; J Monod
Journal: Biochemistry Date: 1968-01 Impact factor: 3.162

2. Fine structure of the gradient of polarity in the z gene of the lac operon of Escherichia coli.

Authors: D Zipser; S Zabell; J Rothman; T Grodzicker; M Wenk
Journal: J Mol Biol Date: 1970-04-14 Impact factor: 5.469

3. Steady-state measurement of the turnover of amino acid in the cellular proteins of growing Escherichia coli: existence of two kinetically distinct reactions.

Authors: M J Pine
Journal: J Bacteriol Date: 1970-07 Impact factor: 3.490

4. A mutation which creates a new site for the re-initiation of polypeptide synthesis in the z gene of the lac operon of Escherichia coli.

Authors: T Grodzicker; D Zipser
Journal: J Mol Biol Date: 1968-12 Impact factor: 5.469

5. Recombinant rabbit secretory immunoglobulin molecules: alpha chains with maternal (paternal) variable-region allotypes and paternal (maternal) constant-region allotypes.

Authors: K L Knight; T R Malek; W C Hanly
Journal: Proc Natl Acad Sci U S A Date: 1974-04 Impact factor: 11.205

6. Polypeptide splicing.

Authors:
Journal: Proc Natl Acad Sci U S A Date: 1976-02 Impact factor: 11.205

6 in total

In vivo splicing of protein: one continuous polypeptide from two independently functioning operons.

1. On the determination of molecular weight of proteins and protein subunits in the presence of 6 M guanidine hydrochloride.

2. Fine structure of the gradient of polarity in the z gene of the lac operon of Escherichia coli.

3. Steady-state measurement of the turnover of amino acid in the cellular proteins of growing Escherichia coli: existence of two kinetically distinct reactions.

4. A mutation which creates a new site for the re-initiation of polypeptide synthesis in the z gene of the lac operon of Escherichia coli.

5. Direction of transcription of a regulatory gene in E. coli.

6. Mutational alteration of the maximal level of Lac operon expression.

7. On the subunit structure of wild-type versus complemented beta-galactosidase of Escherichia coli.

8. Re-initiation of polypeptide synthesis and polarity in the lac operon of Escherichia coli.

9. Mapping of polypeptide reinitiation sites within the beta-galactosidase structural gene.

10. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

1. The synthetase gene of the RNA phages R17, MS2 and f2 has a single UAG terminator codon.

2. Complementation in vitro between mutationally altered beta2 subunits of Escherichia coli tryptophan synthetase.

3. Structural proteins of intracisternal A-particles: possible repetitive sequences.

4. Protein fusion: a novel reaction in bacteriophage lambda head assembly.

5. Recombinant rabbit secretory immunoglobulin molecules: alpha chains with maternal (paternal) variable-region allotypes and paternal (maternal) constant-region allotypes.

6. Polypeptide splicing.