Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 In vitro repression of transcription of the tryptophan operon by trp repressor.

Literature DB >> 4579009

In vitro repression of transcription of the tryptophan operon by trp repressor.

Abstract

The in vitro repression of transcription of the tryptophan operon by the trp repressor from Escherichia coli was studied. By measuring the inhibitory effect for trp-specific RNA synthesis in an in vitro transcription system directed by DNA of trp-transducing phage, we have detected and concentrated a trp repressor in an eluate of a Phi80 ptED native DNA-cellulose column. The repression of transcription of trp operon required the addition of L-tryptophan in the system, and when several tryptophan analogues were added, the repression or derepression was similar to that observed in vivo. The repressor fraction was separated from the majority of tryptophanyl-tRNA synthetase activity by Bio-gel P60 column chromatography.

Entities: Chemical Species

Mesh：

Substances：

Year: 1973 PMID： 4579009 PMCID： PMC433649 DOI： 10.1073/pnas.70.7.1990

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

26 in total

1. A CHEMICAL BASIS FOR THE HOST-INDUCED MODIFICATION OF T-EVEN BACTERIOPHAGES.

Authors: A SHEDLOVSKY; S BRENNER
Journal: Proc Natl Acad Sci U S A Date: 1963-08 Impact factor: 11.205

2. Transduction and recombination study of linkage relationships among the genes controlling tryptophan synthesis in Escherichia coli.

Authors: C YANOFSKY; E S LENNOX
Journal: Virology Date: 1959-08 Impact factor: 3.616

3. Acetylornithinase of Escherichia coli: partial purification and some properties.

Authors: H J VOGEL; D M BONNER
Journal: J Biol Chem Date: 1956-01 Impact factor: 5.157

4. Regulatory mechanism of the tryptophan operon in Escherichia coli: possible interaction between trpR and trpS gene products.

Authors: K Ito
Journal: Mol Gen Genet Date: 1972

10. Tryptophanyl transfer ribonucleic acid synthetase of Escherichia coli. II. Molecular weight, subunit structure, sulfhydryl content, and substrate-binding properties.

Authors: D R Joseph; K H Muench
Journal: J Biol Chem Date: 1971-12-25 Impact factor: 5.157

12 in total

1. Cloning the trpR gene.

Authors: W Roeder; R L Somerville
Journal: Mol Gen Genet Date: 1979-11

2. Tryptophan biosynthesis in Salmonella typhimurium: location in trpB of a genetic difference between strains LT2 and LT7.

Authors: C Stuttard
Journal: J Bacteriol Date: 1975-09 Impact factor: 3.490

3. Regulation of enzyme synthesis in the tryptophan pathway of Acinetobacter calcoaceticus.

Authors: W Cohn; I P Crawford
Journal: J Bacteriol Date: 1976-07 Impact factor: 3.490

4. Transcription of the argF and argI genes of the arginine biosynthetic regulon of Escherichia coli K12, performed in vitro.

Authors: D Sens; W Natter; R T Garvin; E James
Journal: Mol Gen Genet Date: 1977-09-21

9. Molecular dynamics studies of a DNA-binding protein: 2. An evaluation of implicit and explicit solvent models for the molecular dynamics simulation of the Escherichia coli trp repressor.

Authors: J Guenot; P A Kollman
Journal: Protein Sci Date: 1992-09 Impact factor: 6.725

10. Analysis in vivo of factors affecting the control of transcription initiation at promoters containing target sites for trp repressor.

Authors: G Bogosian; R L Somerville
Journal: Mol Gen Genet Date: 1984

In vitro repression of transcription of the tryptophan operon by trp repressor.

1. A CHEMICAL BASIS FOR THE HOST-INDUCED MODIFICATION OF T-EVEN BACTERIOPHAGES.

2. Transduction and recombination study of linkage relationships among the genes controlling tryptophan synthesis in Escherichia coli.

3. Acetylornithinase of Escherichia coli: partial purification and some properties.

4. Regulatory mechanism of the tryptophan operon in Escherichia coli: possible interaction between trpR and trpS gene products.

5. Operator mutants of the tryptophan operon in Escherichia coli.

6. Sequential derepression and repression of the tryptophan operon in E. coli.

7. The periodicity of RNA polymerase initiations: a new regulatory feature of transcription.

8. Direction of in vivo degradation of tryptophan messenger RNA--a correction.

9. Regulation of the enzymes of the tryptophan pathway in Escherichia coli.

10. Tryptophanyl transfer ribonucleic acid synthetase of Escherichia coli. II. Molecular weight, subunit structure, sulfhydryl content, and substrate-binding properties.

1. Cloning the trpR gene.

2. Tryptophan biosynthesis in Salmonella typhimurium: location in trpB of a genetic difference between strains LT2 and LT7.

3. Regulation of enzyme synthesis in the tryptophan pathway of Acinetobacter calcoaceticus.

4. Transcription of the argF and argI genes of the arginine biosynthetic regulon of Escherichia coli K12, performed in vitro.

Review 5. Role of ribonucleic acid polymerase in gene selection in procaryotes.

Review 6. Gene rearrangements in the evolution of the tryptophan synthetic pathway.

7. Interaction of the operator of the tryptophan operon with repressor.

8. DNA sequence of the E. coli trpR gene and prediction of the amino acid sequence of Trp repressor.

9. Molecular dynamics studies of a DNA-binding protein: 2. An evaluation of implicit and explicit solvent models for the molecular dynamics simulation of the Escherichia coli trp repressor.

10. Analysis in vivo of factors affecting the control of transcription initiation at promoters containing target sites for trp repressor.