Literature DB >> 4578944

Competitive inhibition and substrate activity of uridine diphosphate 6-deoxygalactose for Escherichia coli uridine diphosphate galactose 4-epimerase.

M Spencer, P Blackburn, W Ferdinand, G M Blackburn.   

Abstract

UDP-6-deoxygalactose inhibits the UDP-galactose 4-epimerase (EC 5.1.3.2) from Escherichia coli in a competitive manner with respect to the substrate UDP-galactose, giving K(i) 1.3x10(-3)m. As a substrate for the enzyme, it is transformed into UDP-6-deoxyglucose, although the reaction stops before equilibrium is attained. Possible causes of this behaviour are discussed.

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Year:  1973        PMID： 4578944      PMCID： PMC1177484          DOI： 10.1042/bj1310421

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  8 in total

1.  THE ENZYMES OF THE GALACTOSE OPERON IN ESCHERICHIA COLI. I. PURIFICATION AND CHARACTERIZATION OF URIDINE DIPHOSPHOGALACTOSE 4-EPIMERASE.

Authors:  D B WILSON; D S HOGNESS
Journal:  J Biol Chem       Date:  1964-08       Impact factor: 5.157

2.  Synthesis and proof of structure of tritiated glucose and its use in the study of the mechanism of UDPGal-C-4-epimerase.

Authors:  R D BEVILL; J H NORDIN; F SMITH; S KIRKWOOD
Journal:  Biochem Biophys Res Commun       Date:  1963-07-18       Impact factor: 3.575

3.  The determination of enzyme inhibitor constants.

Authors:  M DIXON
Journal:  Biochem J       Date:  1953-08       Impact factor: 3.857

4.  HEREDITARY DEFECTS IN GALACTOSE METABOLISM IN ESCHERICHIA COLI MUTANTS, I. DETERMINATION OF ENZYME ACTIVITIES.

Authors:  H M Kalckar; K Kurahashi; E Jordan
Journal:  Proc Natl Acad Sci U S A       Date:  1959-12       Impact factor: 11.205

5.  The mechanism of action of the enzyme uridine diphosphoglucose 4-epimerase. Proof of an oxidation-reduction mechanism with direct transfer of hydrogen between substrate and the B-position of the enzyme-bound pyridine nucleotide.

Authors:  G L Nelsestuem; S Kirkwood
Journal:  J Biol Chem       Date:  1971-12-25       Impact factor: 5.157

6.  Changes in the specific activities of the galactose enzymes in E. coli under different growth conditions.

Authors:  E Jordan; H Saedler; J Lengeler; P Starlinger
Journal:  Mol Gen Genet       Date:  1967

7.  [The interaction of UDPG-4-epimerase with UDP-deoxyhexoses].

Authors:  T N Druzhinina; M A Novikova; V N Shibaev
Journal:  Biokhimiia       Date:  1969 May-Jun

8.  A vacuum system for the synthesis of nucleotide sugars on a micro scale.

Authors:  J H Nordin; W L Salo; R D Bevill; S Kirkwood
Journal:  Anal Biochem       Date:  1965-12       Impact factor: 3.365
  8 in total
  1 in total

1.  The concerted inactivation of Escherichia coli uridine diphosphate galactose 4-epimerase by sugar nucleotide together with a free sugar.

Authors:  P Blackburn; W Ferdinand
Journal:  Biochem J       Date:  1976-05-01       Impact factor: 3.857
  1 in total

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