Literature DB >> 4578761

A procedure for the rapid purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.

P Humphries, D J McConnell, R L Gordon.   

Abstract

A rapid procedure involving DNA-cellulose chromatography followed either by sedimentation in a high-salt glycerol gradient or by gel filtration is described for the complete purification of Escherichia coli DNA-dependent RNA polymerase.

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Year:  1973        PMID： 4578761      PMCID： PMC1177684          DOI： 10.1042/bj1330201

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  9 in total

1.  ENZYMATIC SYNTHESIS OF RIBONUCLEIC ACID. I. PREPARATION OF RIBONUCLEIC ACID POLYMERASE FROM EXTRACTS OF MICROCOCCUS LYSODEIKTICUS.

Authors:  T NAKAMOTO; C F FOX; S B WEISS
Journal:  J Biol Chem       Date:  1964-01       Impact factor: 5.157

2.  Deoxyribo ucleic acid-directed synthesis of ribonucleic acid by an enzyme from Escherichia coli.

Authors:  M CHAMBERLIN; P BERG
Journal:  Proc Natl Acad Sci U S A       Date:  1962-01-15       Impact factor: 11.205

3.  Studies on deoxyribonucleic acid-dependent ribonucleic acid polymerase from Escherichia coli. Variations of the enzyme activity during growth.

Authors:  K A Abraham; K J Andersen; A Rognes
Journal:  Biochem J       Date:  1972-09       Impact factor: 3.857

4.  Preparation of highly purified ribonucleic acid polymerase; separation from polynucleotide phosphorylase and polyphosphate kinase.

Authors:  D J McConnell; J Bonner
Journal:  Biochemistry       Date:  1972-11-07       Impact factor: 3.162

5.  The role of deoxyribonucleic acid in ribonucleic acid synthesis. XVI. The purification and properties of ribonucleic acid polymerase from yeast: preferential utilization of denatured deoxyribonucleic acid as template.

Authors:  E W Frederick; U Maitra; J Hurwitz
Journal:  J Biol Chem       Date:  1969-01-25       Impact factor: 5.157

6.  Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels.

Authors:  A L Shapiro; E Viñuela; J V Maizel
Journal:  Biochem Biophys Res Commun       Date:  1967-09-07       Impact factor: 3.575

7.  Studies with DNA-cellulose chromatography. I. DNA-binding proteins from Escherichia coli.

Authors:  B M Alberts; F J Amodio; M Jenkins; E D Gutmann; F L Ferris
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1968

8.  The isolation and the characterization of DNA region bound to Escherichia coli RNA polymerase.

Authors:  A Matsukage; S Murakami; T Kameyama
Journal:  Biochim Biophys Acta       Date:  1969-03-18

9.  A deoxyribonucleic acid polymerase from Micrococcus luteus (Micrococcus lysodeikticus) isolated on deoxyribonucleic acid-cellulose.

Authors:  R M Litman
Journal:  J Biol Chem       Date:  1968-12-10       Impact factor: 5.157
  9 in total
  8 in total

1.  Isolation and characterization of nucleoid proteins from Escherichia coli.

Authors:  K Yamazaki; A Nagata; Y Kano; F Imamoto
Journal:  Mol Gen Genet       Date:  1984

2.  Conformational variation in superhelical deoxyribonucleic acid.

Authors:  A M Campbell
Journal:  Biochem J       Date:  1978-04-01       Impact factor: 3.857

3.  The firA gene, a locus involved in the expression of rifampicin resistance in Escherichia coli. II. Characterisation of bacterial proteins coded by lambdafirA transducing phages.

Authors:  R Lathe; J P Lecocq
Journal:  Mol Gen Genet       Date:  1977-07-07

4.  Characterization of adenovirus type 2 transcriptional complexes isolated from infected HeLa cell nuclei.

Authors:  J Wilhelm; O Brison; C Kedinger; P Chambon
Journal:  J Virol       Date:  1976-07       Impact factor: 5.103

5.  Purification of Escherichia coli RNA polymerase using a self-cleaving elastin-like polypeptide tag.

Authors:  Baley A Fong; Alison R Gillies; Iraj Ghazi; Gary LeRoy; Kathleen C Lee; Lars F Westblade; David W Wood
Journal:  Protein Sci       Date:  2010-06       Impact factor: 6.725

6.  Comparative studies of RNA polymerase subunits from various bacteria.

Authors:  R Fukuda; A Ishihama; T Saitoh; M Taketo
Journal:  Mol Gen Genet       Date:  1977-07-20

7.  Promoter selectivity of Escherichia coli RNA polymerase. II: Altered promoter selection by mutant holoenzymes.

Authors:  T Nomura; A Ishihama; M Kajitani; T Takahashi; N Nakada; K Yoshinaga
Journal:  Mol Gen Genet       Date:  1984

8.  Efficient and selective initiation by yeast RNA polymerase B in a dinucleotide-primed reaction.

Authors:  B Lescure; V Williamson; A Sentenac
Journal:  Nucleic Acids Res       Date:  1981-01-10       Impact factor: 16.971
  8 in total

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