Literature DB >> 4577138

A stopped-flow apparatus with light-scattering detection and its application to biochemical reactions.

D Riesner, H Buenemann.   

Abstract

A stopped-flow apparatus utilizing light-scattering for following the progress of a reaction is described. The method is applicable to all reactions that result in a significant change of the average molecular weight. It was possible due to several modifications of a conventional stopped-flow system to obtain a sensitivity comparable to that of commercial instruments for static light-scattering measurements. Experiments on three reactions are reported: association and dissociation of mercury ligands with DNA, dissociation of the dimers of DNA-dependent RNA polymerase, and complex formation of tRNA(Ser) (yeast) with the cognate aminoacyl-tRNA synthetase. The changes in the intensities of the scattered light are calculated and compared with the measured amplitudes.

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Year:  1973        PMID： 4577138      PMCID： PMC433382          DOI： 10.1073/pnas.70.3.890

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  10 in total

1.  The reversible reaction of Hg (II) and double-stranded polynucleotides. A step-function theory and its significance.

Authors:  S KATZ
Journal:  Biochim Biophys Acta       Date:  1963-02-26

2.  Observations on the use of phenol for the isolation of deoxyribonucleic acid.

Authors:  J S COLTER; R A BROWN; K A ELLEM
Journal:  Biochim Biophys Acta       Date:  1962-01-22

3.  SIMPLIFIED ROTATING CYLINDER VISCOMETER FOR DNA.

Authors:  B H Zimm; D M Crothers
Journal:  Proc Natl Acad Sci U S A       Date:  1962-06       Impact factor: 11.205

4.  THE PROPERTIES OF SONIC FRAGMENTS OF DEOXYRIBOSE NUCLEIC ACID.

Authors:  P Doty; B B McGill; S A Rice
Journal:  Proc Natl Acad Sci U S A       Date:  1958-05       Impact factor: 11.205

5.  Studies on the conformation of DNA-dependent RNA polymerase in solution by small-angle x-ray measurements.

Authors:  I Pilz; O Kratky; D Rabussay
Journal:  Eur J Biochem       Date:  1972-07-13

6.  Chromatography of rNA polymerase from escherichia coli on single stranded DNA-agarose columns.

Authors:  C Nüsslein; B Heyden
Journal:  Biochem Biophys Res Commun       Date:  1972-04-14       Impact factor: 3.575

7.  Characterization of the native and denatured conformations of tRNA Ser and tRNA Phe from yeast.

Authors:  R E Streeck; H G Zachau
Journal:  Eur J Biochem       Date:  1972-10

8.  A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.

Authors:  R R Burgess
Journal:  J Biol Chem       Date:  1969-11-25       Impact factor: 5.157

9.  Kinetics of sodium dodecyl sulfate solubilization of Mycoplasma laidlawii plasma membranes.

Authors:  J J Auborn; E M Eyring; G L Choules
Journal:  Proc Natl Acad Sci U S A       Date:  1971-09       Impact factor: 11.205

10.  Apparatus for rapid and sensitive spectrophotometry.

Authors:  Q H Gibson; L Milnes
Journal:  Biochem J       Date:  1964-04       Impact factor: 3.857
  10 in total
  1 in total

1.  Stopped-flow Light Scattering Analysis of Red Blood Cell Glycerol Permeability.

Authors:  Patrizia Gena; Piero Portincasa; Sabino Matera; Yonathan Sonntag; Michael Rützler; Giuseppe Calamita
Journal:  Bio Protoc       Date:  2020-08-20
  1 in total

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