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Literature DB >> 457676

Analytical peptide mapping by high performance liquid chromatography. Application to intestinal calcium-binding proteins.

Abstract

Peptide mapping of underivatized tryptic digests of bovine and chick intestinal calcium-binding proteins has been accomplished by high performance liquid chromatography (HPLC). High precision analysis of nanomolar quantities of peptides were achieved in less than 1 h (recycle time). Peak resolution and definition are superior compared to conventional techniques and recoveries of both small (4-residue) hydrophilic and large (30-residue) hydrophobic peptides are excellent. The total amino acid composition of the bovine intestinal calcium-binding protein has been accounted for on the basis of two tryptic maps of 20 microgram of protein each.

Entities: Chemical Species

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Year: 1979 PMID： 457676

Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157

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Cited

17 in total

1. Bacteriophage HK97 structure: wholesale covalent cross-linking between the major head shell subunits.

Authors: M P Popa; T A McKelvey; J Hempel; R W Hendrix
Journal: J Virol Date: 1991-06 Impact factor: 5.103

2. Structural studies of a carbohydrate-containing immunoglobulin-lambda-light-chain amyloid-fibril protein (AL) of variable subgroup III.

Authors: E Holm; K Sletten; G Husby
Journal: Biochem J Date: 1986-11-01 Impact factor: 3.857

3. Amino acid sequence and comparative antigenicity of chicken metallothionein.

Authors: C C McCormick; C S Fullmer; J S Garvey
Journal: Proc Natl Acad Sci U S A Date: 1988-01 Impact factor: 11.205

4. Identification of a thrombin sequence with growth factor activity on macrophages.

Authors: R Bar-Shavit; A J Kahn; K G Mann; G D Wilner
Journal: Proc Natl Acad Sci U S A Date: 1986-02 Impact factor: 11.205

5. The amino acid sequence of a carbohydrate-containing immunoglobulin-light-chain-type amyloid-fibril protein.

Authors: T Tveteraas; K Sletten; P Westermark
Journal: Biochem J Date: 1985-11-15 Impact factor: 3.857

6. Strategy for the mass spectrometric verification and correction of the primary structures of proteins deduced from their DNA sequences.

Authors: B W Gibson; K Biemann
Journal: Proc Natl Acad Sci U S A Date: 1984-04 Impact factor: 11.205

Analytical peptide mapping by high performance liquid chromatography. Application to intestinal calcium-binding proteins.

1. Bacteriophage HK97 structure: wholesale covalent cross-linking between the major head shell subunits.

2. Structural studies of a carbohydrate-containing immunoglobulin-lambda-light-chain amyloid-fibril protein (AL) of variable subgroup III.

3. Amino acid sequence and comparative antigenicity of chicken metallothionein.

4. Identification of a thrombin sequence with growth factor activity on macrophages.

5. The amino acid sequence of a carbohydrate-containing immunoglobulin-light-chain-type amyloid-fibril protein.

6. Strategy for the mass spectrometric verification and correction of the primary structures of proteins deduced from their DNA sequences.

7. Mutations in the uncE gene affecting assembly of the c-subunit of the adenosine triphosphatase of Escherichia coli.

8. Sequence of the 20-kilodalton heavy chain peptide from the carboxyl-terminus of bovine cardiac myosin subfragment-1.

9. Chicken intestinal 28-kilodalton calbindin-D: complete amino acid sequence and structural considerations.

10. Characterization of the binding of the anti-sickling compound, BW12C, to haemoglobin.