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Literature DB >> 4576022

Topographical location of H-Y antigen on mouse spermatozoa by immunoelectronmicroscopy.

G C Koo, C W Stackpole, E A Boyse, U Hämmerling, M P Lardis.

Abstract

H-Y (male) antigen was visually located on mouse sperm by electron microscopy, by use of the indirect hybrid antibody method and tobacco mosaic virus as the visual marker. Labeling was achieved by centrifugation of the sperm through a discontinuous gradient consisting of alternating layers of immune reagents and wash solutions. Treated sperm were examined topographically by preparation of platinum-carbon replicas. Antigen was located mainly on the acrosomal cap of the sperm head.

Entities: Chemical Species

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Year: 1973 PMID： 4576022 PMCID： PMC433529 DOI： 10.1073/pnas.70.5.1502

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

13 in total

1. A comparative view of sperm ultrastructure.

Authors: D W Fawcett
Journal: Biol Reprod Suppl Date: 1970

2. Another visual marker of antibody for electron microscopy.

Authors: T Aoki; H A Wood; L J Old; E A Boyse; E De Harven; M P Lardis; C W Stackpole
Journal: Virology Date: 1971-09 Impact factor: 3.616

3. Serological demonstration of H-Y (male) antigen on mouse sperm.

Authors: E H Goldberg; E A Boyse; D Bennett; M Scheid; E A Carswell
Journal: Nature Date: 1971-08-13 Impact factor: 49.962

4. A freeze-etching study of rabbit spermatozoa with particular reference to head structures.

Authors: J K Koehler
Journal: J Ultrastruct Res Date: 1970-12

5. Substructure of the mammalian acrosome.

Authors: D M Phillips
Journal: J Ultrastruct Res Date: 1972-03

6. Synthetic activities during spermatogenesis in the mouse RNA and protein.

Authors: V Monesi
Journal: Exp Cell Res Date: 1965-08 Impact factor: 3.905

7. The genetics of the mammalian gamete.

Authors: R A Beatty
Journal: Biol Rev Camb Philos Soc Date: 1970-02

8. Detection of H-2 antigens on mouse spermatozoa by the cytotoxicity test.

Authors: E H Goldberg; T Aoki; E A Boyse; D Bennett
Journal: Nature Date: 1970-11-07 Impact factor: 49.962

9. Serologically demonstrable alloantigens of mouse epidermal cells.

Authors: M Scheid; E A Boyse; E A Carswell; L J Old
Journal: J Exp Med Date: 1972-04-01 Impact factor: 14.307

10. Use of hybrid antibody with anti-gamma-G and anti-ferritin specificities in locating cell surface antigens by electron microscopy.

Authors: U Hämmerling; T Aoki; E de Harven; E A Boyse; L J Old
Journal: J Exp Med Date: 1968-12-01 Impact factor: 14.307

23 in total

1. Identification of a male-specific (H-Y) antigen on the flagellar plasma membrane of ram epididymal spermatozoa.

Authors: M P Bradley; I T Forrester; B F Heslop
Journal: Hum Genet Date: 1987-04 Impact factor: 4.132

2. A hormone-like action of H-Y antigen and gonadal development of XY/XX mosaic males and hermaphrodites.

Authors: S Ohno
Journal: Hum Genet Date: 1976-12-29 Impact factor: 4.132

3. Application of monoclonal anti-HY antibody for human H-Y typing.

Authors: G C Koo; N Tada; R Chaganti; U Hammerling
Journal: Hum Genet Date: 1981 Impact factor: 4.132

4. Radioimmunoassay reveals no anti-male specificity in putative H-Y antisera.

Authors: D N Crichton; C M Steel
Journal: Clin Exp Immunol Date: 1980-12 Impact factor: 4.330

5. Evidence for postmeiotic expression of ribosomal RNA genes during male gametogenesis.

Authors: M Schmid; F J Hofgärtner; M T Zenzes; W Engel
Journal: Hum Genet Date: 1977-10-14 Impact factor: 4.132

6. The distribution of sex-specific (H-Y) antigens within the seminiferous tubules of the testis: an immunohistochemical study.

Authors: M P Bradley; B F Heslop
Journal: Hum Genet Date: 1988-08 Impact factor: 4.132

10. Characterization of the antigen recognized by a monoclonal antibody MN9: unique transport pathway to the equatorial segment of sperm head during spermiogenesis.

Authors: K Toshimori; I Tanii; S Araki; C Oura
Journal: Cell Tissue Res Date: 1992-12 Impact factor: 5.249