| Literature DB >> 4573826 |
Abstract
Six solutes known to be actively transported by bacteria were studied with the cell in a "minimum energy" state to determine if sufficient energy were available from cellular stores of ATP to supply the energy necessary to run postulated membrane-situated "pumps." Steady-state cellular concentrations of potassium, calcium, magnesium, leucine, glycine, and alpha-methyl glucoside were determined together with tracer fluxes, oxygen consumption, ATP turnover, and the P:O ratio. From these measurements, it was calculated that the energy supply, 4.20 cal/340 min-g dry wt, fell far short of the energy necessary (28.28 cal/340 min-g dry wt), by classical membrane theory, to operate "pumps."Entities:
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Year: 1973 PMID: 4573826 PMCID: PMC1484216 DOI: 10.1016/S0006-3495(73)85977-6
Source DB: PubMed Journal: Biophys J ISSN: 0006-3495 Impact factor: 4.033