| Literature DB >> 4572710 |
J Sebastian, M M Bhargava, H O Halvorson.
Abstract
Two deoxyribonucleic acid (DNA)-dependent ribonucleic acid (RNA) polymerases (I, II) have been solubilized from isolated Saccharomyces cerevisiae nuclei. The enzymes can be separated by chromatography on O-diethylaminoethyl Sephadex. Both enzymes are active with high-molecular-weight nuclear yeast DNA, although RNA polymerase I has a higher affinity for polydeoxy-adenylic-thymidylic acid and RNA polymerase II for denatured DNA. RNA polymerase I is active only with manganese. alpha-Amanitin inhibits only the activity of RNA polymerase II.Entities:
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Year: 1973 PMID: 4572710 PMCID: PMC251732 DOI: 10.1128/jb.114.1.1-6.1973
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490