Literature DB >> 4570610

Evidence for the existence of two arginyl-transfer ribonucleic acid synthetase activities in Escherichia coli.

D W Yem, L S Williams.   

Abstract

Two arginyl-transfer ribonucleic acid (tRNA) synthetase (EC 6.1.1.13, arginine: ribonucleic acid ligase adenosine monophosphate) activities were found in extracts of Escherichia coli strains AB1132 and NP2. The two arginyl-tRNA synthetase activities in extracts of strain AB1132 were found to be separable by diethylaminoethyl-cellulose column chromatography, Sephadex column fractionation, and by sucrose density gradient centrifugation. In addition, in the standard assay using extracts of strain AB1132 there were two pH optima for arginyl-tRNA synthetase activity. Furthermore, when arginyl-tRNA synthetase of strain NP2 was fractionated by hydroxylapatite column chromatography, two activities were observed which were similar to those of strain AB1132.

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Year:  1973        PMID： 4570610      PMCID： PMC285305          DOI： 10.1128/jb.113.2.891-894.1973

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  18 in total

1.  Two enzymatically active forms of lysyl-tRNA synthetase from E. coli B.

Authors:  L L. Kisselev; I D. Baturina
Journal:  FEBS Lett       Date:  1972-05-01       Impact factor: 4.124

2.  Transduction of linked genetic characters of the host by bacteriophage P1.

Authors:  E S LENNOX
Journal:  Virology       Date:  1955-07       Impact factor: 3.616

3.  Glycyl-tRNA synthetase: an oligomeric protein containing dissimilar subunits.

Authors:  D L Ostrem; P Berg
Journal:  Proc Natl Acad Sci U S A       Date:  1970-12       Impact factor: 11.205

4.  The subunit structure of phenylalanyl-tRNA synethetase of Escherichia coli.

Authors:  M H Kosakowski; A Böck
Journal:  Eur J Biochem       Date:  1970-01

5.  Map location of arginyl-tRNA synthetase mutations in Escherichia coli K-12.

Authors:  P H Cooper; I N Hirshfield; W K Maas
Journal:  Mol Gen Genet       Date:  1969-08-15

6.  Phage-induced modification of valyl-tRNA synthetase.

Authors:  F C Neidhardt; G L Marchin; W H McClain; R F Boyd; C F Earhart
Journal:  J Cell Physiol       Date:  1969-10       Impact factor: 6.384

7.  Binding of transfer RNA and arginine to the arginine transfer RNA synthetase of Escherichia coli.

Authors:  S K Mitra; K Chakraburtty; A H Mehler
Journal:  J Mol Biol       Date:  1970-04-14       Impact factor: 5.469

8.  Prolyl transfer ribonucleic acid synthetase of Escherichia coli. I. Purification and evidence for subunits.

Authors:  M L Lee; K H Muench
Journal:  J Biol Chem       Date:  1969-01-25       Impact factor: 5.157

9.  Studies on valyl-tRNA synthetase and tRNA from Escherichia coli. I. Purification and properties of the enzyme from normal Escherichia coli strains.

Authors:  M Yaniv; F Gros
Journal:  J Mol Biol       Date:  1969-08-28       Impact factor: 5.469

10.  The biochemical characterization of two mutant arginyl transfer ribonucleic acid synthetases from Escherichia coli K-12.

Authors:  I N Hirshfield; H P Bloemers
Journal:  J Biol Chem       Date:  1969-06-10       Impact factor: 5.157
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  3 in total

1.  Thermosensitive mutants of Escherichia coli K-12 altered in the catalytic Subunit and in a Regulatory factor of the glutamy-transfer ribonucleic acid synthetase.

Authors:  J Lapointe; G Delcuve
Journal:  J Bacteriol       Date:  1975-05       Impact factor: 3.490

2.  Studies on the regulation of the branched chain amino acyl-tRNA synthetases of the fungusNeurospora crassa.

Authors:  I V Economidis; R P Wagner
Journal:  Wilehm Roux Arch Dev Biol       Date:  1980-10

3.  Genetic location of two mutations affecting the lysyl-transfer ribonucleic acid synthetase of Bacillus subtilis.

Authors:  F M Racine; W Steinberg
Journal:  J Bacteriol       Date:  1974-10       Impact factor: 3.490
  3 in total

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