Literature DB >> 4570279

Radiobacteriolysis: a new technique using chromium-51 for assaying anti-Vibrio cholerae antibodies.

U Blachman, W R Clark, M J Pickett.   

Abstract

A new method for detecting and quantitating antibodies against Vibrio cholerae is described. The reaction involves the release of radiochromium from prelabeled vibrios in the presence of specific antibody and complement. The entire assay can be completed within 5 hr. The method is highly reproducible, immunologically specific, temperature- and complement-dependent, and significantly more sensitive than other methods currently used for titration of anti-Vibrio cholerae antibodies. The technique is also potentially applicable to titration of antibodies against other gram-negative bacteria.

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Year:  1973        PMID: 4570279      PMCID: PMC422638          DOI: 10.1128/iai.7.1.53-61.1973

Source DB:  PubMed          Journal:  Infect Immun        ISSN: 0019-9567            Impact factor:   3.441


  17 in total

1.  QUANTITATIVE TITRATIONS OF MOUSE H-2 ANTIBODIES USING CR-51-LABELLED TARGET CELLS.

Authors:  H WIGZELL
Journal:  Transplantation       Date:  1965-05       Impact factor: 4.939

2.  Quantitative studies on the bactericidal actions of serum and complement. I. A rapid photometric growth assay for bactericidal activity.

Authors:  L H MUSCHEL; H P TREFFERS
Journal:  J Immunol       Date:  1956-01       Impact factor: 5.422

3.  The tagging of red cells and plasma proteins with radioactive chromium.

Authors:  S J GRAY; K STERLING
Journal:  J Clin Invest       Date:  1950-12       Impact factor: 14.808

4.  Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs.

Authors:  K T Brunner; J Mauel; J C Cerottini; B Chapuis
Journal:  Immunology       Date:  1968-02       Impact factor: 7.397

5.  A kinetic study of the bacteriolytic and bactericidal action of human serum.

Authors:  A A Glynn; C M Milne
Journal:  Immunology       Date:  1967-06       Impact factor: 7.397

6.  Lysozyme and serum bactericidal action.

Authors:  D Gemsa; S D Davis; R J Wedgwood
Journal:  Nature       Date:  1966-05-28       Impact factor: 49.962

7.  Vibriocidal test and its parameters.

Authors:  R Ganguly; B C Deb; D L Shrivastava
Journal:  Indian J Med Res       Date:  1969-05       Impact factor: 2.375

8.  Vibriocidal and agglutinating antibody patterns in cholera patients.

Authors:  R B Sack; D Barua; R Saxena; C C Carpenter
Journal:  J Infect Dis       Date:  1966-12       Impact factor: 5.226

9.  Normal serum cytotoxicity for P32-labeled smooth Enterobacteriaceae. I. Loss of label, death, and ultrastructural damage.

Authors:  J K Spitznagel; L A Wilson
Journal:  J Bacteriol       Date:  1966-01       Impact factor: 3.490

10.  Serological studies in cholera. 2. The vibriocidal antibody response of cholera patients determined by a microtechnique.

Authors:  A S Benenson; A Saad; W H Mosley
Journal:  Bull World Health Organ       Date:  1968       Impact factor: 9.408

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  5 in total

1.  Simple method for purifying choleragenoid, the natural toxoid of Vibrio cholerae.

Authors:  J J Mekalanos; R J Collier; W R Romig
Journal:  Infect Immun       Date:  1977-06       Impact factor: 3.441

2.  In-111 Pseudomonas aeruginosa: a simple method of labeling live bacteria with a gamma-emitting radioisotope.

Authors:  K Bettin; M O Allen; D N Gerding; L Forstrom; R Shafer
Journal:  Eur J Nucl Med       Date:  1986

Review 3.  Bactericidal and bacteriolytic activity of serum against gram-negative bacteria.

Authors:  P W Taylor
Journal:  Microbiol Rev       Date:  1983-03

4.  Experimental Cholera in Chinchillas: the Immune Response in Serum and Intestinal Secretions to Vibrio cholerae and Cholera Toxin.

Authors:  U Blachman; S R Graboff; G E Haag; E Gottfeld; M J Pickett
Journal:  Infect Immun       Date:  1974-11       Impact factor: 3.441

5.  Genetic contributors to risk of schizophrenia in the presence of a 22q11.2 deletion.

Authors:  Isabelle Cleynen; Worrawat Engchuan; Matthew S Hestand; Tracy Heung; Aaron M Holleman; H Richard Johnston; Thomas Monfeuga; Donna M McDonald-McGinn; Raquel E Gur; Bernice E Morrow; Ann Swillen; Jacob A S Vorstman; Carrie E Bearden; Eva W C Chow; Marianne van den Bree; Beverly S Emanuel; Joris R Vermeesch; Stephen T Warren; Michael J Owen; Pankaj Chopra; David J Cutler; Richard Duncan; Alex V Kotlar; Jennifer G Mulle; Anna J Voss; Michael E Zwick; Alexander Diacou; Aaron Golden; Tingwei Guo; Jhih-Rong Lin; Tao Wang; Zhengdong Zhang; Yingjie Zhao; Christian Marshall; Daniele Merico; Andrea Jin; Brenna Lilley; Harold I Salmons; Oanh Tran; Peter Holmans; Antonio Pardinas; James T R Walters; Wolfram Demaerel; Erik Boot; Nancy J Butcher; Gregory A Costain; Chelsea Lowther; Rens Evers; Therese A M J van Amelsvoort; Esther van Duin; Claudia Vingerhoets; Jeroen Breckpot; Koen Devriendt; Elfi Vergaelen; Annick Vogels; T Blaine Crowley; Daniel E McGinn; Edward M Moss; Robert J Sharkus; Marta Unolt; Elaine H Zackai; Monica E Calkins; Robert S Gallagher; Ruben C Gur; Sunny X Tang; Rosemarie Fritsch; Claudia Ornstein; Gabriela M Repetto; Elemi Breetvelt; Sasja N Duijff; Ania Fiksinski; Hayley Moss; Maria Niarchou; Kieran C Murphy; Sarah E Prasad; Eileen M Daly; Maria Gudbrandsen; Clodagh M Murphy; Declan G Murphy; Antonio Buzzanca; Fabio Di Fabio; Maria C Digilio; Maria Pontillo; Bruno Marino; Stefano Vicari; Karlene Coleman; Joseph F Cubells; Opal Y Ousley; Miri Carmel; Doron Gothelf; Ehud Mekori-Domachevsky; Elena Michaelovsky; Ronnie Weinberger; Abraham Weizman; Leila Kushan; Maria Jalbrzikowski; Marco Armando; Stéphan Eliez; Corrado Sandini; Maude Schneider; Frédérique Sloan Béna; Kevin M Antshel; Wanda Fremont; Wendy R Kates; Raoul Belzeaux; Tiffany Busa; Nicole Philip; Linda E Campbell; Kathryn L McCabe; Stephen R Hooper; Kelly Schoch; Vandana Shashi; Tony J Simon; Flora Tassone; Celso Arango; David Fraguas; Sixto García-Miñaúr; Jaume Morey-Canyelles; Jordi Rosell; Damià H Suñer; Jasna Raventos-Simic; Michael P Epstein; Nigel M Williams; Anne S Bassett
Journal:  Mol Psychiatry       Date:  2020-02-03       Impact factor: 15.992

  5 in total

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