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Literature DB >> 4569402

Plasmid-associated functions of a stable Flac.

Abstract

Several functions associated with the stable plasmid, FlacS, have been examined. Our results indicate that the sex pili synthesized by Escherichia coli strains carrying FlacS are altered in some manner as evidenced by a very inefficient adsorption of male-specific phages. On the other hand, FlacS-mediated entry exclusion of related plasmids and plasmid incompatibility function(s) appear normal. The presence of covalently closed circular deoxyribonucleic acid in E. coli strains harboring FlacS indicates that it is an autonomously replicating plasmid. Based on beta-galactosidase levels and the percentage of covalently closed circular deoxyribonucleic acid, it appears that the stability of the FlacS is not the result of multiple copies of this plasmid. FlacS appears larger than its precursor, F(ts114)lac, in sedimentation through alkaline sucrose gradients.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1973 PMID： 4569402 PMCID： PMC251616 DOI： 10.1128/jb.113.1.183-191.1973

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

31 in total

1. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

2. Chromosomal mutations affecting the stability of sex-factors in Escherichia coli.

Authors: H Yamagata; H Uchida
Journal: J Mol Biol Date: 1972-01-28 Impact factor: 5.469

3. Physical properties and mechanism of transfer of R factors in Escherichia coli.

Authors: D Vapnek; M B Lipman; W D Rupp
Journal: J Bacteriol Date: 1971-10 Impact factor: 3.490

4. Studies on Escherichia coli sex factors. 3. Covalently closed F'Lac DNA molecules.

Authors: D Freifelder
Journal: J Mol Biol Date: 1968-05-28 Impact factor: 5.469

5. Derepression of F-lac in Salmonella typhimurium by a determinant for kanamycin resistance.

Authors: H R Smith; J N Grindley; N D Grindley; E S Anderson
Journal: Genet Res Date: 1970-12 Impact factor: 1.588

6. The problems of drug-resistant pathogenic bacteria. The replication of R-factor DNA in Escherichia coli K-12 following conjugation.

Authors: S Falkow; L S Tompkins; R P Silver; P Guerry; D J Le Blanc
Journal: Ann N Y Acad Sci Date: 1971-06-11 Impact factor: 5.691

7. Circular DNA forms of colicinogenic factors E1, E2 and E3 from Escherichia coli.

Authors: M Bazaral; D R Helinski
Journal: J Mol Biol Date: 1968-09-14 Impact factor: 5.469

8. T7 translational control mechanisms and their inhibiton by F factors.

Authors: T G Morrison; M H Malamy
Journal: Nat New Biol Date: 1971-05-12

Review 9. Circular DNA.

Authors: D R Helinski; D B Clewell
Journal: Annu Rev Biochem Date: 1971 Impact factor: 23.643

4. Isolation of a hybrid F' factor-carrying Escherichia coli lactose region and Salmonella typhimurium histidine region, F42-400 (F' ts114 lac+, his+): its partial characterization and behavior in Salmonella typhimurium.

Authors: R N Rao; M G Pereira
Journal: J Bacteriol Date: 1975-09 Impact factor: 3.490

5. Evidence for a composite state of an F'his, gnd element and a cryptic plasmid in a derivative of Salmonella typhimurium LT2.

Authors: D R Oliver; J J Manis; H J Whitfield
Journal: J Bacteriol Date: 1974-07 Impact factor: 3.490

5 in total