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Literature DB >> 4566214

Bacterial conjugation: an analysis of mixed recombinant clones.

Abstract

A fraction of recombinant colonies resulting from conjugation is heterogenetic for unselected markers. Constitutivity for alkaline phosphatase synthesis (phoR) is studied as the unselected marker. The frequency of phoR heterogeneity depends on the genetic distance between phoR and the selected marker. Various models are considered which explain the formation of heterogenetic colonies (mixed clones), and experiments are described which test these models. It is concluded that the Hfr fragment can replicate and participate more than once in recombination thus yielding heterogenetic colonies.

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Substances：

Year: 1972 PMID： 4566214 PMCID： PMC1212837

Source DB: PubMed Journal: Genetics ISSN： 0016-6731 Impact factor: 4.562

9 in total

1. Genetic control of repression of alkaline phosphatase in E. coli.

Authors: H ECHOLS; A GAREN; S GAREN; A TORRIANI
Journal: J Mol Biol Date: 1961-08 Impact factor: 5.469

2. Inactivation of chromosomal fragments transferred from hfr strains.

Authors: T Itoh; J Tomizawa
Journal: Genetics Date: 1971-05 Impact factor: 4.562

Review 3. The mechanism of genetic recombination.

Authors: H L Whitehouse
Journal: Biol Rev Camb Philos Soc Date: 1970-05

4. Incorporation of 5-bromodeoxyuridine into DNA of wild type Escherichia coli and its use for the enrichment of auxotrophic mutants.

Authors: A Rosner; E Yagil
Journal: Mol Gen Genet Date: 1970

8 in total

1. Recombinant clone heterogeneity in Escherichia coli conjunction: effect of pH and partially replicated recipient deoxyribonucleic acid.

Authors: J T Ou
Journal: Genetics Date: 1975-07 Impact factor: 4.562

2. Mechanisms of recombination by the RecBC and the RecF pathways following conjugation in Escherichia coli K12.

Authors: S K Mahajan; A R Datta
Journal: Mol Gen Genet Date: 1979-01-16

3. Mutants of Escherichia coli K-12 "cryptic," or deficient in 5'-nucleotidase (uridine diphosphate-sugar hydrolase) and 3'-nucleotidase (cyclic phosphodiesterase) activity.

Authors: I R Beacham; R Kahana; L Levy; E Yagil
Journal: J Bacteriol Date: 1973-11 Impact factor: 3.490

Bacterial conjugation: an analysis of mixed recombinant clones.

1. Genetic control of repression of alkaline phosphatase in E. coli.

2. Inactivation of chromosomal fragments transferred from hfr strains.

Review 3. The mechanism of genetic recombination.

4. Incorporation of 5-bromodeoxyuridine into DNA of wild type Escherichia coli and its use for the enrichment of auxotrophic mutants.

5. Deoxyribonucleic acid transferred from ultraviolet-irradiated excision-defective Hfr cells of Escherichia coli K-12.

6. Asymmetric transfer of DNA strands in bacterial conjugation.

7. Genetic recombination in Escherichia coli: clone heterogeneity and the kinetics of segregation.

8. DNA transfer in bacterial conjugation.

9. A proposal for a uniform nomenclature in bacterial genetics.

1. Recombinant clone heterogeneity in Escherichia coli conjunction: effect of pH and partially replicated recipient deoxyribonucleic acid.

2. Mechanisms of recombination by the RecBC and the RecF pathways following conjugation in Escherichia coli K12.

3. Mutants of Escherichia coli K-12 "cryptic," or deficient in 5'-nucleotidase (uridine diphosphate-sugar hydrolase) and 3'-nucleotidase (cyclic phosphodiesterase) activity.

4. Conjugational recombination in Escherichia coli: genetic analysis of recombinant formation in Hfr x F- crosses.

5. Single-stranded conjugation in Escherichia coli K-12.

6. Suppression of the formation of polygenotypic recombinant colonies by a maf mutation in mating with HfrH.

7. Homologous Recombination-Experimental Systems, Analysis, and Significance.

Review 8. The process of general recombination in Escherichia coli K-12: structure of intermediate products.