Literature DB >> 4559831

Purification of sex pili from Escherichia coli carrying a derepressed F-like R factor.

J P Beard, T G Howe, M H Richmond.   

Abstract

A procedure for the purification of sex pili is described. Escherichia coli K-12 carrying Rldrd19 was grown in nutrient broth and blended at the time of peak sex pilus production. The cells were removed by centrifugation, and the supernatant fraction was concentrated, dialyzed, and clarified in an ultrafiltration system. After an additional blend and a clearing spin, the material was centrifuged in a CsCl gradient, and the fractions containing the sex pili were subjected to isoelectric focusing. About 5 mg of intact pili of approximately 98% purity were obtained by this method from about 100 g (wet weight) of cells.

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Year:  1972        PMID： 4559831      PMCID： PMC251357          DOI： 10.1128/jb.111.3.814-820.1972

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  17 in total

1.  Stages in phage R17 infection. II. Ionic requirements for phage R17 attachment to F-pili.

Authors:  R E Danziger; W Paranchych
Journal:  Virology       Date:  1970-03       Impact factor: 3.616

2.  Repressor-minus and operator-constitutive de-repressed mutants of F-like R factors: their effect on chromosomal transfer by HfrC.

Authors:  E Meynell; M Cooke
Journal:  Genet Res       Date:  1969-12       Impact factor: 1.588

Review 3.  Phylogenetic relationships of drug-resistance factors and other transmissible bacterial plasmids.

Authors:  E Meynell; G G Meynell; N Datta
Journal:  Bacteriol Rev       Date:  1968-03

4.  Functions of F pili in mating-pair formation and male bacteriophage infection studies by blending spectra and reappearance kinetics.

Authors:  C Novotny; E Raizen; W S Knight; C C Brinton
Journal:  J Bacteriol       Date:  1969-06       Impact factor: 3.490

Review 5.  The structure, function, synthesis and genetic control of bacterial pili and a molecular model for DNA and RNA transport in gram negative bacteria.

Authors:  C C Brinton
Journal:  Trans N Y Acad Sci       Date:  1965-06

6.  General properties of F-pili.

Authors:  L W Wendt; K A Ippen; R Valentine
Journal:  Biochem Biophys Res Commun       Date:  1966-05-25       Impact factor: 3.575

7.  Morphological features of the pili associated with Escherichia coli K 12 carrying R factors or the F factor.

Authors:  A M Lawn
Journal:  J Gen Microbiol       Date:  1966-11

8.  An assay for the male substance (F-pili) of Escherichia coli K-12.

Authors:  K A Ippen; R C Valentine
Journal:  Biochem Biophys Res Commun       Date:  1965-10-08       Impact factor: 3.575

9.  Studies on extracellular proteins from Staphylococcus aureus. IV. Separation of alpha-toxin by isoelectric focusing.

Authors:  T Wadström
Journal:  Biochim Biophys Acta       Date:  1968-10-21

10.  Isoelectric fractionation, analysis, and characterization of ampholytes in natural pH gradients. IV. Further studies on the resolving power in connection with separation of myoglobins.

Authors:  O Vesterberg; H Svensson
Journal:  Acta Chem Scand       Date:  1966
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  9 in total

1.  Detection of a protein, similar to the sex pilus subunit, in the outer membrane of Escherichia coli cells carrying a derepressed F-like R factor.

Authors:  J P Beard; J C Connolly
Journal:  J Bacteriol       Date:  1975-04       Impact factor: 3.490

2.  Role of the cell surface in bacterial mating: requirement for intact mucopeptide in donors for the expression of surface exclusion in R+ strains of Escherichia coli.

Authors:  J P Beard; S F Bishop
Journal:  J Bacteriol       Date:  1975-09       Impact factor: 3.490

3.  Caulobacter crescentus pili: structure and stage-specific expression.

Authors:  C Lagenaur; N Agabian
Journal:  J Bacteriol       Date:  1977-07       Impact factor: 3.490

4.  Cell-cell interactions in conjugating Escherichia coli: purification of F pili with biological activity.

Authors:  R Helmuth; M Achtman
Journal:  Proc Natl Acad Sci U S A       Date:  1978-03       Impact factor: 11.205

5.  Male-specific bacteriophage MS2 propagation in fluorophenylalanine-resistant Escherichia coli K12.

Authors:  S T Jenkins; J P Beard; T G Howe
Journal:  J Virol       Date:  1974-07       Impact factor: 5.103

6.  Characterization of a translocation unit encoding resistance to mercuric ions that occurs on a nonconjugative plasmid in Pseudomonas aeruginosa.

Authors:  V A Stanisich; P M Bennett; M H Richmond
Journal:  J Bacteriol       Date:  1977-03       Impact factor: 3.490

7.  Composition and molecular weight of pili purified from Pseudomonas aeruginosa K.

Authors:  L S Frost; W Paranchych
Journal:  J Bacteriol       Date:  1977-07       Impact factor: 3.490

8.  Relation between F, R1, R100 and R144 Escherichia coli K-12 donor strains in mating.

Authors:  L Havekes; W Hoekstra; H Kempen
Journal:  Mol Gen Genet       Date:  1977-10-20

9.  Transformation in Escherichia coli: stages in the process.

Authors:  H E Bergmans; I M van Die; W P Hoekstra
Journal:  J Bacteriol       Date:  1981-05       Impact factor: 3.490
  9 in total

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